Fig. 1

Expression and identification of fusion proteins. a SDS–PAGE analysis of CSFV-E2 protein expression and purification. 1- supernatant from uninduced bacteria; 2- supernatant from induced bacteria; 3- inclusion bodies from uninduced bacteria; 4- inclusion bodies from induced bacteria; 5- empty vector control; 6- purified E2 protein. b SDS–PAGE analysis of PPV-VP2 protein expression and purification. 1- supernatant from uninduced bacteria; 2-supernatant from induced bacteria; 3- inclusion bodies from uninduced bacteria; 4- inclusion bodies from induced bacteria; 5- empty vector control; 6- purified VP2 protein. c SDS–PAGE analysis of JEV-EDIII protein expression and purification. 1- supernatant from uninduced bacteria; 2- supernatant from induced bacteria; 3- inclusion bodies from uninduced bacteria; 4- inclusion bodies from induced bacteria; 5- empty vector control: 6- purified EDIII protein. d SDS-PAGE analysis of PRRSV-N protein expression and purification. 1- supernatant from uninduced bacteria; 2- supernatant from induced bacteria; 3- inclusion bodies from uninduced bacteria; 4- inclusion bodies from induced bacteria; 5. Control from empty vector; 6- purified N protein. e Western blot of bacterial lysates. After SDS-PAGE, the purified proteins were subject to western blotting using 6 × His-tagged mAb as secondary antibody.1. E2 protein; 2. VP2 protein; 3. EDIII protein; 4. N protein; 5. empty vector control