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Table 2 Description of primers used

From: High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system

Targeted trichostrongyloid species; primer nameSequence 5′ to 3′Size [base pairs]Tm [°C]Reference
Haemonchus contortus
 HcPy2PCR ForGAC GCA TTC ACT TGG AGG AG40053[24]
 HcPy2PCR RevBiotin-CAT AGG TTG GAT TTG TGA GTT
Teladorsagia circumcincta
 F200Y_FORBiotin- ACC TTA CAA TGC CAC TCT TTC TG10058[62]
 F200Y_REVGCG GAA GCA GAT ATC GTA CAG
 F167Y_FORGCA TTC TTT GGG AGG AGG TA20058
 F167Y_REVBiotin-TGC ACC TCG AGA ACC TGT ACA TA
Trichostrongylus colubriformis
 FORTAC CCA GAT CGG ATT ATG TCT TC 58[25]
 REVBiotin – GGC AAG TCG TGA CAC CAG ACA
Pyrosequencing primer
 Hc200PySeq1 (Haemonchus, Codon 200)TAG AGA ACA CCG ATG AAANot applicable[24]
 T. circ. F200Y_SEQ (Teladorsagia, Codon 200)RGA GCY TCA TTA TCG ATR[62]
 T. circ. F167Y_SEQ (Teladorsagia, Codon 167)CGG ATA GAA TCA TGG CT[62]
T. colubriformis Codon 198_200GCA GTA CTC GTG TC[25]
T. colubriformis Codon 167CGG ATA GAA TCA TGG CT
  1. Abbreviations
  2. AR Anthelmintic resistanceBZ Benzimidazole anthelminticsGIN Gastrointestinal nematodesSNP Single nucleotide polymorphismML Macrocyclic lactonesFECRT Faecal egg count reduction testEHT Egg hatch test
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