High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system

Table 2 Description of primers used

Targeted trichostrongyloid species; primer name	Sequence 5′ to 3′	Size [base pairs]	Tm [°C]	Reference
Haemonchus contortus
HcPy2PCR For	GAC GCA TTC ACT TGG AGG AG	400	53	[24]
HcPy2PCR Rev	Biotin-CAT AGG TTG GAT TTG TGA GTT	400	53	[24]
Teladorsagia circumcincta
F200Y_FOR	Biotin- ACC TTA CAA TGC CAC TCT TTC TG	100	58	[62]
F200Y_REV	GCG GAA GCA GAT ATC GTA CAG	100	58
F167Y_FOR	GCA TTC TTT GGG AGG AGG TA	200	58
F167Y_REV	Biotin-TGC ACC TCG AGA ACC TGT ACA TA	200	58
Trichostrongylus colubriformis
FOR	TAC CCA GAT CGG ATT ATG TCT TC		58	[25]
REV	Biotin – GGC AAG TCG TGA CAC CAG ACA		58	[25]
Pyrosequencing primer
Hc200PySeq1 (Haemonchus, Codon 200)	TAG AGA ACA CCG ATG AAA	Not applicable		[24]
T. circ. F200Y_SEQ (Teladorsagia, Codon 200)	RGA GCY TCA TTA TCG ATR			[62]
T. circ. F167Y_SEQ (Teladorsagia, Codon 167)	CGG ATA GAA TCA TGG CT			[62]
T. colubriformis Codon 198_200	GCA GTA CTC GTG TC			[25]
T. colubriformis Codon 167	CGG ATA GAA TCA TGG CT			[25]

Abbreviations
AR Anthelmintic resistanceBZ Benzimidazole anthelminticsGIN Gastrointestinal nematodesSNP Single nucleotide polymorphismML Macrocyclic lactonesFECRT Faecal egg count reduction testEHT Egg hatch test

ISSN: 1746-6148