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Fig. 3 | BMC Veterinary Research

Fig. 3

From: MRI perfusion analysis using freeware, standard imaging software

Fig. 3

Step-by-step description how signal intensities can be extracted from perfusion-weighted images using Horos®. Horos does not provide a ‘ROI enhancement’ PlugIn, but it is nevertheless possible to obtain dynamic perfusion data. First, the respective sequences must be opend using the 2D-Viewer (a). Second, a ROI is drawn around the anatomy of interest (small pink rectangle visible on (b)). Importantly, the ROI must be drawn on the first dynamic image showing the anatomic region of interest. We decided for a rectangular ROI for the AIF and the pencil tool for the anatomic regions (b). Third, one chooses the option ‘propagate selected ROI’ from the drop down menu ‘ROI’ (c). The ROI must be propagated up to the last dynamic image of the respective slice (therefore you need to start at the first slice). In our case, we obtained 40 dynamic images per slice, thus starting at slice 81 we need to propagate the ROI up to slice number 120 (d). Fourth the options ‘ROI tools’ and ‘Export ROI’ need to be selected from the ‘PlugIn’ drop down menu (e). This allows for an export of the data as a CSV file (f)

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