Table 3 Primers and probes used for real-time PCR
From: Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya
Target | Gene targeted | Sequences of primers and probes (5′ -3′) | Fluorophore/ quencher | Reference |
|---|---|---|---|---|
Genus Brucella | IS711 | Forward GGCCTACCGCTGCGAAT Reverse TTGCGGACAGTCACCATAATG Probe AAGCCAACACCCGGC | FAM/−MGBNFQ | Matero, 2011 [13] |
Genus Brucella | Bcsp31 | Forward GCTCGGTTGCCAATATCAATGC Reverse GGGTAAAGCGTCGCCAGAAG Probe AAATCTTCCACCTTGCCCTTGCCATCA | 6-FAM/BHQ1 | Probert, 2004 [14] |
B. abortus | IS711 downstream of alkB | Forward GCGGCTTTTCTATCACGGTATTC Reverse CATGCGCTATGATCTGGTTACG Probe CGCTCATGCTCGCCAGACTTCAATG | JOE/BHQ1 | |
B. melitensis | IS711 downstream of BMEI1162 | Forward AACAAGCGGCACCCCTAAAA Reverse CATGCGCTATGATCTGGTTACG Probe CAGGAGTGTTTCGGCTCAGAATAATCCACA | Texas Red/BHQ2 |