Table 2 The primers and probe for conventional PCR and LFD-RPA assay used in this assay
Assay | Primers and probe | Sequences 5′-3’ | Amplicon size (bp) |
|---|---|---|---|
RPA | N-F | CGTCGTAAGACCCAGCATCAAGCTCCCAAGCGGAC | 257 |
N-R | GATTATGCTGTACCCTCGATCGT | ||
LFD-RPA | LFD-F | CGTCGTAAGACCCAGCATCAAGCTCCCAAGCGGAC | 257 |
LFD-R | Biotin-GACTGTGATTGAGTAGGAGAAGGTAAGGGTAATTG | ||
LFD-Probe | FITC-GTCGGCTCTGGAGACACTGAGAAGACGGGT (THF)ATGGCTGATCCTCGCATCA-C3-Spacer |