Fig. 1From: Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstickOptimization of reaction temperature and time of the recombinase polymerase amplification combined with a lateral flow dipstick (LFD-RPA) assay using 1 × 107 copies/μL of recombinant plasmid DNA as a template. The top line on the lateral flow dipstick is the control line, and the next line is the test line. a Evaluation of different reaction temperatures from 4 to 42 °C for 20 min. b Evaluation of different reaction times between 0 and 30 min at 37 °CBack to article page