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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick

Fig. 1

Optimization of reaction temperature and time of the recombinase polymerase amplification combined with a lateral flow dipstick (LFD-RPA) assay using 1 × 107 copies/μL of recombinant plasmid DNA as a template. The top line on the lateral flow dipstick is the control line, and the next line is the test line. a Evaluation of different reaction temperatures from 4 to 42 °C for 20 min. b Evaluation of different reaction times between 0 and 30 min at 37 °C

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