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Table 2 Strains and plasmids

From: Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice

Strain or plasmidRelevant characteristics or genotypeSource or reference
E. coli strains
 BL21FpT hsdSB (rB mBal dcm (DE3)Invitrogen
 χ7213thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu pir; KmrGift from Dr. Curtiss, III
Salmonella Choleraesuis
 C78–3Wild type, virulent, CVCC79103Lab stock
 C500S. Choleraesuis vaccine strain attenuated by chemical mutation, CVCC79500[29]
 rSC0005Δpmi-2426, C78–3[9]
 rSC0008Δpmi-2426 ΔrelA199::araC PBADlacI TTThis study
 rSC0009Δpmi-2426 ΔrelA199::araC PBADlacI TT
ΔPfur88::TT araC PBADfur
This study
 rSC0011ΔPcrp527::TT araC PBADcrp Δpmi-2426
ΔrelA199::araC PBADlacI TT ΔasdA33
 rSC0012Δpmi-2426 ΔrelA199::araC PBADlacI TT
ΔPfur88::TT araC PBADfur ΔasdA33
This study
 C78–3 ΔPfur88::TT araC PBADfurC78–3
 C78–3 ΔPcrp527::TT araC PBADcrpC78–3
Streptococcus suis serotype 2Wild type, virulent, CVCC3928Lab stock
 pYA3493Asd+; pBR ori, Ptrc promoter, β-lactamase signal sequence-based periplasmic secretion plasmid[15]
 pS-SaoApYA3493 with SaoA, Ptrc promoter[5]
 pET28aexpression vector, T7 promoter; KmrNovagen
Suicide vector
 pRE112sacB mobRP4 R6K oriV oriT Cmr[30]
 pDMS197tetrsacB mobRP4 R6K oriV oriT[30]
 pYA3832ΔPcrp527::TT araC PBADcrp, pRE112[31]
 pYA3546Δpmi-2426, pDMS197[31]
 pS003ΔrelA199::araC PBADlacI TT, pRE112[9]
 pS005ΔPfur88::TT araC PBADfurThis study
 pYA3736ΔasdA33, pRE112[9]