Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice

Table 2 Strains and plasmids

Strain or plasmid	Relevant characteristics or genotype	Source or reference
E. coli strains
BL21	F⁻pT hsdSB (rB⁻ mB⁻al dcm (DE3)	Invitrogen
χ7213	thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu pir; Km^r	Gift from Dr. Curtiss, III
Salmonella Choleraesuis
C78–3	Wild type, virulent, CVCC79103	Lab stock
C500	S. Choleraesuis vaccine strain attenuated by chemical mutation, CVCC79500	[29]
rSC0005	Δpmi-2426, C78–3	[9]
rSC0008	Δpmi-2426 ΔrelA199::araC P_BADlacI TT	This study
rSC0009	Δpmi-2426 ΔrelA199::araC P_BADlacI TT ΔP_fur88::TT araC P_BADfur	This study
rSC0011	ΔP_crp527::TT araC P_BADcrp Δpmi-2426 ΔrelA199::araC P_BADlacI TT ΔasdA33	[9]
rSC0012	Δpmi-2426 ΔrelA199::araC P_BADlacI TT ΔP_fur88::TT araC P_BADfur ΔasdA33	This study
rSC0018	ΔasdA33	C500
	C78–3 ΔP_fur88::TT araC P_BADfur	C78–3
	C78–3 ΔP_crp527::TT araC P_BADcrp	C78–3
Streptococcus suis serotype 2	Wild type, virulent, CVCC3928	Lab stock
Plasmids
pYA3493	Asd⁺; pBR ori, P_trc promoter, β-lactamase signal sequence-based periplasmic secretion plasmid	[15]
pS-SaoA	pYA3493 with SaoA, P_trc promoter	[5]
pET28a	expression vector, T7 promoter; Km^r	Novagen
Suicide vector
pRE112	sacB mobRP4 R6K oriV oriT Cm^r	[30]
pDMS197	tet^rsacB mobRP4 R6K oriV oriT	[30]
pYA3832	ΔP_crp527::TT araC P_BADcrp, pRE112	[31]
pYA3546	Δpmi-2426, pDMS197	[31]
pS003	ΔrelA199::araC P_BADlacI TT, pRE112	[9]
pS005	ΔP_fur88::TT araC P_BADfur	This study
pYA3736	ΔasdA33, pRE112	[9]

ISSN: 1746-6148