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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice

Fig. 1

Diagram of chromosomal mutation and phenotypes of the S. Choleraesuis vaccine strain rSC0012. (a) Schematic map of ΔPfur::TT araC PBADfur deletion - insertion mutation; (b) Regulated decreased synthesis of Fur (ΔPfur::TT araC PBADfur) and regulated delayed synthesis of SaoA in rSC0012(pS-SaoA) with the ΔrelA::araC PBADlacI TT mutation. The rSC0012(pS-SaoA) strain was grown in NB with arabinose (Lane 1) when OD600 reach 0.8 and then diluted at a 1:10 ratio into fresh NB without arabinose. The process continued for 4 times (Lane 2- 5); each lane was loaded around 4.6 × 107 CFU cells. Synthesis of Fur and SaoA were detected by western blot using corresponding antiserum. M: protein marker; (c) LPS profile of Δpmi mutation in rSC0012 in NB grown with or without 0.2% mannose. Lanes: 1, wild type C78-3; 2, C500; 3, rSC0012 with mannose; 4, rSC0012 without mannose; (d) Growth curves of rSC0012 with and without DAP, rSC0012(pYA3493) or rSC0012(pS-SaoA) in LB were measured with a spectrophotometer (OD600) at the indicated time intervals

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