Fig. 1From: Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in miceDiagram of chromosomal mutation and phenotypes of the S. Choleraesuis vaccine strain rSC0012. (a) Schematic map of ΔPfur::TT araC PBADfur deletion - insertion mutation; (b) Regulated decreased synthesis of Fur (ΔPfur::TT araC PBADfur) and regulated delayed synthesis of SaoA in rSC0012(pS-SaoA) with the ΔrelA::araC PBADlacI TT mutation. The rSC0012(pS-SaoA) strain was grown in NB with arabinose (Lane 1) when OD600 reach 0.8 and then diluted at a 1:10 ratio into fresh NB without arabinose. The process continued for 4 times (Lane 2- 5); each lane was loaded around 4.6 × 107 CFU cells. Synthesis of Fur and SaoA were detected by western blot using corresponding antiserum. M: protein marker; (c) LPS profile of Δpmi mutation in rSC0012 in NB grown with or without 0.2% mannose. Lanes: 1, wild type C78-3; 2, C500; 3, rSC0012 with mannose; 4, rSC0012 without mannose; (d) Growth curves of rSC0012 with and without DAP, rSC0012(pYA3493) or rSC0012(pS-SaoA) in LB were measured with a spectrophotometer (OD600) at the indicated time intervalsBack to article page