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Table 3 pH, gut histology and immunohistochemistry parameters at different intestinal levels

From: Dietary supplementation of micro-encapsulated sodium butyrate in healthy horses: effect on gut histology and immunohistochemistry parameters

 

Butyrate

Placebo

P - value

Mean

SD

Mean

SD

Treatment

Period

Age

Sex

Treatment x Period

pH

 D

5.96

0.90

6.39

0.28

0.119

0.308

0.36

0.149

0.053

 J

7.20

0.52

7.46

0.21

0.166

0.442

0.385

0.803

0.225

 I

7.58

0.22

7.49

0.25

0.110

0.143

0.66

0.418

0.061

 CAE

6.74

0.15

6.67

0.14

0.820

0.047

0.746

0.961

0.768

 LVC

6.61

0.20

6.64

0.24

0.697

0.001

0.174

0.966

0.467

RDC

6.56

0.24

6.55

0.10

0.375

0.165

0.142

0.852

0.583

Crypt depth (μm)

CAE

262

33

262

34

0.143

0.195

0.822

0.916

0.118

LVC

318

42

320

41

0.262

0.662

0.731

0.488

0.251

RDC

399

94

518

84

< 0.001

0.314

0.798

< 0.001

0.002

Area % of CD3 positive cells

D

10.096

5.131

6.193

4.535

0.745

0.010

0.802

0.127

0.192

J

8.306

6.108

12.367

7.903

0.241

0.051

0.253

0.503

0.282

I

9.964

6.782

14.451

10.616

0.258

0.036

0.313

0.733

0.283

CAE

16.562

10.081

16.945

9.767

0.482

0.151

0.924

0.682

0.433

 LVC

11.585

8.596

12.441

4.212

0.301

0.407

0.293

0.598

0.407

 RDC

11.126

7.560

14.240

6.977

0.207

0.173

0.398

0.769

0.254

Area % of CD20 positive cells

 D

6.571

7.349

5.519

4.850

0.232

0.004

0.235

0.076

0.085

 J

3.798

4.169

2.279

1.585

0.241

0.051

0.253

0.503

0.282

 I

9.428

11.087

3.143

2.409

0.714

0.529

0.761

0.021

0.840

 CAE

2.427

2.981

2.720

2.110

0.759

0.159

0.505

0.079

0.704

 LVC

3.186

3.127

2.452

2.854

0.153

0.068

0.008

0.032

0.034

 RDC

2.950

3.022

3.240

2.448

0.940

0.324

0.510

0.785

0.805

Number of KI67 positive cells per crypt

 RDC

25

10

32

12

0.650

0.984

0.687

0.632

0.972

  1. D duodenum, J jejunum, I ileum, CAE cecum, LVC left ventral colon, RDC right dorsal colon