Table 2 Comparative performances of RT-PCR and Real-time RT-qPCR assays for detection of aerosol specimens
From: Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay
Herd No. | Size | PI positivity | Sero-positivity | Samples background | Number of samples | RT-PCR | Real-time qPCR | ||
|---|---|---|---|---|---|---|---|---|---|
P | N | P | N | ||||||
A | 700 | 2.4% | 37% | Milking parlour | 7 | 1 | 6 | 3 | 4 |
Cowshed | 7 | 1 | 6 | 2 | 5 | ||||
Cattle playground | 8 | 0 | 8 | 0 | 8 | ||||
B | 168 | 1.2% | 24% | Milking parlour | 12 | 1 | 11 | 1 | 11 |
Cowshed | 6 | 1 | 5 | 1 | 5 | ||||
Cattle playground | 9 | 0 | 9 | 0 | 9 | ||||
C | 306 | 3.0% | 43% | Milking parlour | 12 | 1 | 11 | 3 | 9 |
Cowshed | 10 | 1 | 9 | 3 | 7 | ||||
Cattle playground | 12 | 0 | 12 | 0 | 12 | ||||
D | 209 | 2.9% | 27% | Milking parlour | 9 | 0 | 9 | 1 | 8 |
Cowshed | 13 | 2 | 11 | 5 | 8 | ||||
Cattle playground | 10 | 0 | 10 | 0 | 10 | ||||
E | 525 | 3.5% | 69% | Milking parlour | 10 | 3 | 7 | 5 | 5 |
Cowshed | 11 | 2 | 9 | 2 | 9 | ||||
Cattle playground | 9 | 0 | 9 | 0 | 9 | ||||
F | 326 | 1.3% | 45% | Milking parlour | 8 | 1 | 7 | 2 | 6 |
Cowshed | 8 | 1 | 7 | 1 | 7 | ||||
Cattle playground | 8 | 0 | 8 | 0 | 8 | ||||
| Â | Â | Â | Â | Total | 169 | 15 | 154 | 29 | 140 |