Antimicrobial resistance and novel mutations detected in the gyrA and parC genes of Pseudomonas aeruginosa strains isolated from companion dogs

Table 4 Comparison of quinolone resistance-determining regions among fluoroquinolone-resistant Pseudomonas aeruginosa between healthy and infected dogs

Strain no.	Amino acid substitution ^a		MIC (μg/mL) ^b
Strain no.	gyrA	parC	CIP	LVX	ENR	MFX
*Healthy group*
PAE18	–	Pro116Arg	4	16	≥16	≥16
PAE43	Thr83Ile	Pro116Arg	2	8	≥16	8
PAE51	–	Pro116Arg	16	16	≥16	≥16
PAE52	Thr83Ile	Ser87Leu, Pro116Arg	≥32	≥32	≥16	≥16
PAE71	–	Pro116Arg	≥32	≥32	≥16	≥16
PAE85	–	Pro116Arg	0.25	0.5	4	1
*Infected group*
KVNON14	Asp87Gly	Pro116Arg	4	8	≥16	≥16
KVNON23	Thr83Ile	Pro116Arg	≥32	≥32	≥16	≥16
KVNON33	Thr83Ile, Asp87Gly	Ser87Leu, Pro116Arg	≥32	≥32	≥16	≥16
KVNON47	–	Pro116fs	0.5	2	4	2
KVNON66	Asp87Gly	Pro116Arg	4	8	≥16	≥16
KVNON127	Asp87Tyr	Pro116Arg	1	2	4	4
KVNON184	–	Pro116Ala	0.125	0.5	8	1
KVNON194	Leu55Gln, Asp82Asn, Thr83Ala	Ser87Leu, Pro116Ala	≥32	≥32	≥16	≥16
KVNON199	Thr83Ile	Pro116Arg	8	32	≥16	≥16
KVNON216	–	Pro116Arg	0.5	2	4	2
KVNON219	Thr83Ile	Pro116Arg	1	4	8	8
KVNON271	–	Pro116Arg	2	4	4	2
KVNON279	Thr83Ile	Pro116Arg	4	8	≥16	≥16
KVNON324	–	Pro116Arg	0.5	4	4	4
KVNON442	Asp87Asn	Pro116Arg	0.5	2	4	4
KVNON508	–	Pro116fs	0.5	2	4	2
KVNON509	–	Pro116Arg	2	2	4	4

^a Amino acid substitutions in gyrA and parC: Thr83Ile, ACC → ATC; Thr83Ala, ACC → GCC; Asp87Gly, GAC → GGC; Leu55Gln, CTG → CAG; Asp82Asn, GAC → AAC; Asp87Asn, GAC → AAC; Asp87Tyr, GAC → TAC; Pro116Arg, CCG → CGG; Pro116Ala, CCG → GCG; Ser87Leu, TGC → TTG; Pro116fs, CGG → CG-. Ala, alanine; Arg, arginine; Asn, asparagine; Asp, aspartic acid; Cys, cysteine; Gln, glutamine; Gly, glycine; Ile, Isoleucine; Leu, leucine; Pro, proline; Ser, serine; Thr, threonine; Tyr, tyrosine; fs, frameshift
^bNAL nalidixic acid, CIP ciprofloxacin, LVX levofloxacin, ENR enrofloxacin, MFX marbofloxacin

ISSN: 1746-6148