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Table 4 Comparison of quinolone resistance-determining regions among fluoroquinolone-resistant Pseudomonas aeruginosa between healthy and infected dogs

From: Antimicrobial resistance and novel mutations detected in the gyrA and parC genes of Pseudomonas aeruginosa strains isolated from companion dogs

Strain no.Amino acid substitution aMIC (μg/mL) b
gyrAparCCIPLVXENRMFX
Healthy group
 PAE18Pro116Arg416≥16≥16
 PAE43Thr83IlePro116Arg28≥168
 PAE51Pro116Arg1616≥16≥16
 PAE52Thr83IleSer87Leu, Pro116Arg≥32≥32≥16≥16
 PAE71Pro116Arg≥32≥32≥16≥16
 PAE85Pro116Arg0.250.541
Infected group
 KVNON14Asp87GlyPro116Arg48≥16≥16
 KVNON23Thr83IlePro116Arg≥32≥32≥16≥16
 KVNON33Thr83Ile, Asp87GlySer87Leu, Pro116Arg≥32≥32≥16≥16
 KVNON47Pro116fs0.5242
 KVNON66Asp87GlyPro116Arg48≥16≥16
 KVNON127Asp87TyrPro116Arg1244
 KVNON184Pro116Ala0.1250.581
 KVNON194Leu55Gln, Asp82Asn, Thr83AlaSer87Leu, Pro116Ala≥32≥32≥16≥16
 KVNON199Thr83IlePro116Arg832≥16≥16
 KVNON216Pro116Arg0.5242
 KVNON219Thr83IlePro116Arg1488
 KVNON271Pro116Arg2442
 KVNON279Thr83IlePro116Arg48≥16≥16
 KVNON324Pro116Arg0.5444
 KVNON442Asp87AsnPro116Arg0.5244
 KVNON508Pro116fs0.5242
 KVNON509Pro116Arg2244
  1. a Amino acid substitutions in gyrA and parC: Thr83Ile, ACC → ATC; Thr83Ala, ACC → GCC; Asp87Gly, GAC → GGC; Leu55Gln, CTG → CAG; Asp82Asn, GAC → AAC; Asp87Asn, GAC → AAC; Asp87Tyr, GAC → TAC; Pro116Arg, CCG → CGG; Pro116Ala, CCG → GCG; Ser87Leu, TGC → TTG; Pro116fs, CGG → CG-. Ala, alanine; Arg, arginine; Asn, asparagine; Asp, aspartic acid; Cys, cysteine; Gln, glutamine; Gly, glycine; Ile, Isoleucine; Leu, leucine; Pro, proline; Ser, serine; Thr, threonine; Tyr, tyrosine; fs, frameshift
  2. bNAL nalidixic acid, CIP ciprofloxacin, LVX levofloxacin, ENR enrofloxacin, MFX marbofloxacin