Fig. 2

Phylogenetic trees based on the full-length nucleotide sequence of different target genes: glycoprotein B (a), thymidine kinase (b) and glycoprotein G (c) from the BoHV-2 ITA/2018/468 strain isolated in the present study or retrieved from International Committee on Taxonomy of Viruses (ICTV) database. Posterior output of the tree was derived from Bayesian inference using 4 chains run for > 1 million generations, a general time-reversible model, a proportion of invariable sites, a gamma distribution of rate variation across sites, and a subsampling frequency of 1000. Posterior probability values > 95% are indicated on the tree nodes. The black arrows indicate the BoHV-2 ITA/2018/468 strain isolated in this study. BoHV-1 (Genus Varicellovirus) strain (NC_001847) was used as an outgroup. Scale bars indicate nucleotide substitutions per site. GB, glycoprotein B; TK, thymidine kinase; GG, glycoprotein G