Skip to main content

Table 1 Results of ROC analysis with cut-offs set in compliance with the technical abilities of the test

From: Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs

Antigen (concentration)Microarray platformSample materialn (pos./neg.)1AUC (95% CI)Cut-offSensitivitySpecificityYouden IndexMethod cut-off
T. gondii (0.5)ArrayTubeserum110 (25/85)0.91 (0.82–1)0.110.840.950.79Youden Max
ArrayTubemeat juice97 (12/85)0.94 (0.85–1)0.10.750.980.73cut-off = 0.1
ArrayStripserum94 (12/82)0.87 (0.73–1)0.10.670.980.65cut-off = 0.1
ArrayStripmeat juice95 (12/83)0.99 (0.98–1)0.10.830.960.79cut-off = 0.1
Y. enterocolitica Yop O:3 (0.5)ArrayTubeserum90 (39/51)0.97 (0.93–1)0.550.870.960.83Youden Max
ArrayTubemeat juice90 (39/51)0.91 (0.85–0.98)0.340.920.840.76Youden Max
ArrayStripserum87 (37/50)0.97 (0.94–1)0.2910.940.94Youden Max
ArrayStripmeat juice88 (38/50)0.94 (0.9–0.99)0.2410.780.78Youden Max
M. hyopneumoniae (1:10)ArrayTubeserum90 (60/30)0.93 (0.88–0.98)0.420.8310.83Youden Max
ArrayTubemeat juice87 (58/29)0.93 (0.87–0.98)0.370.880.930.81Youden Max
ArrayStripserum86 (56/30)0.84 (0.75–0.92)0.10.730.870.6cut-off = 0.1
ArrayStripmeat juice88 (58/30)0.66 (0.55–0.78)0.10.3510.35cut-off = 0.1
PRRSV in-house mix (0.75)ArrayTubeserum90 (64/26)0.87 (0.8–0.94)0.230.690.960.65Youden Max
ArrayTubemeat juice90 (64/26)0.88 (0.82–0.95)0.10.4410.44cut-off = 0.1
ArrayStripserum87 (64/23)0.69 (0.57–0.8)0.190.420.960.38Youden Max
ArrayStripmeat juice88 (64/24)0.88 (0.8–0.96)0.10.550.960.51cut-off = 0.1
APP (1:5)ArrayTubeserum90 (56/34)0.75 (0.64–0.85)0.170.610.590.2Se Min 0.6
ArrayTubemeat juice89 (56/33)0.77 (0.67–0.87)0.170.80.610.41Youden Max
ArrayStripserum87 (56/31)0.71 (0.6–0.82)0.10.540.870.41cut-off = 0.1
ArrayStripmeat juice87 (55/32)0.78 (0.68–0.87)0.10.60.840.44Se Min 0.6
  1. 1 Number of positive and negative samples in ROC analysis