Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs

Loreck, Katharina; Mitrenga, Sylvia; Heinze, Regina; Ehricht, Ralf; Engemann, Claudia; Lueken, Caroline; Ploetz, Madeleine; Greiner, Matthias; Meemken, Diana

doi:10.1186/s12917-020-02308-4

Table 1 Results of ROC analysis with cut-offs set in compliance with the technical abilities of the test

From: Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs

Antigen (concentration)	Microarray platform	Sample material	n (pos./neg.)¹	AUC (95% CI)	Cut-off	Sensitivity	Specificity	Youden Index	Method cut-off
T. gondii (0.5)	ArrayTube	serum	110 (25/85)	0.91 (0.82–1)	0.11	0.84	0.95	0.79	Youden Max
	ArrayTube	meat juice	97 (12/85)	0.94 (0.85–1)	0.1	0.75	0.98	0.73	cut-off = 0.1
	ArrayStrip	serum	94 (12/82)	0.87 (0.73–1)	0.1	0.67	0.98	0.65	cut-off = 0.1
	ArrayStrip	meat juice	95 (12/83)	0.99 (0.98–1)	0.1	0.83	0.96	0.79	cut-off = 0.1
Y. enterocolitica Yop O:3 (0.5)	ArrayTube	serum	90 (39/51)	0.97 (0.93–1)	0.55	0.87	0.96	0.83	Youden Max
	ArrayTube	meat juice	90 (39/51)	0.91 (0.85–0.98)	0.34	0.92	0.84	0.76	Youden Max
	ArrayStrip	serum	87 (37/50)	0.97 (0.94–1)	0.29	1	0.94	0.94	Youden Max
	ArrayStrip	meat juice	88 (38/50)	0.94 (0.9–0.99)	0.24	1	0.78	0.78	Youden Max
M. hyopneumoniae (1:10)	ArrayTube	serum	90 (60/30)	0.93 (0.88–0.98)	0.42	0.83	1	0.83	Youden Max
	ArrayTube	meat juice	87 (58/29)	0.93 (0.87–0.98)	0.37	0.88	0.93	0.81	Youden Max
	ArrayStrip	serum	86 (56/30)	0.84 (0.75–0.92)	0.1	0.73	0.87	0.6	cut-off = 0.1
	ArrayStrip	meat juice	88 (58/30)	0.66 (0.55–0.78)	0.1	0.35	1	0.35	cut-off = 0.1
PRRSV in-house mix (0.75)	ArrayTube	serum	90 (64/26)	0.87 (0.8–0.94)	0.23	0.69	0.96	0.65	Youden Max
	ArrayTube	meat juice	90 (64/26)	0.88 (0.82–0.95)	0.1	0.44	1	0.44	cut-off = 0.1
	ArrayStrip	serum	87 (64/23)	0.69 (0.57–0.8)	0.19	0.42	0.96	0.38	Youden Max
	ArrayStrip	meat juice	88 (64/24)	0.88 (0.8–0.96)	0.1	0.55	0.96	0.51	cut-off = 0.1
APP (1:5)	ArrayTube	serum	90 (56/34)	0.75 (0.64–0.85)	0.17	0.61	0.59	0.2	Se Min 0.6
	ArrayTube	meat juice	89 (56/33)	0.77 (0.67–0.87)	0.17	0.8	0.61	0.41	Youden Max
	ArrayStrip	serum	87 (56/31)	0.71 (0.6–0.82)	0.1	0.54	0.87	0.41	cut-off = 0.1
	ArrayStrip	meat juice	87 (55/32)	0.78 (0.68–0.87)	0.1	0.6	0.84	0.44	Se Min 0.6

¹ Number of positive and negative samples in ROC analysis

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