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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

Fig. 1

West blot results of samples. a showed the results of western blot about Occludin and Claudin-1. After treated for 24 h, various concentrations of betaine (0-2 mmol/L) improve the expression Occludin and Claudin-1 in IPEC-J2 cells. c confirms that LPS down-regulate the protein levels of Occludin and Claudin-1, while betaine totally attenuated the down-regulation and recovered to a normal level. b and d were the statistical graphs of the density ratios of different proteins to β-actin calculated by Image J and analyzed by Graphpad Prism6. All the results were repeated four times (n = 4, *P < 0.05, **P < 0.01 compared with control)

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