Beta-catenin inhibits bovine parainfluenza virus type 3 replication via innate immunity pathway

BMC Veterinary Research

Table 1 List of primers used in this study

Primer name	Sequence (5′-3′)	Application
β-catenin-F	ACTGCTGGGACCTTGCACAA	qRT-PCR to detect β-catenin
β-catenin-R	ACTGGTGAACCGAGCATCTTCA	qRT-PCR to detect β-catenin
IFN-β-F	CCTGTGCCTGATTTCATCATGA	qRT-PCR to detect IFN-β
IFN-β-R	GCAAGCTGTAGCTCCTGGAAAG	qRT-PCR to detect IFN-β
OAS1-F	GATGTCCTGCCCGCCTTT	qRT-PCR to detect OAS1
OAS1-R	GCTGGACGTAGATTTGAGGGTTA	qRT-PCR to detect OAS1
β-actin-F	GATGAGATTGGCATGGCTTTA	qRT-PCR to detect β-actin
β-actin-R	AACCGACTGCTGTCACCTTC	qRT-PCR to detect β-actin
β-catenin-F (Xba I)	GCTCTAGAGCCACCATGGATTACAAGGATGACGACGATAAGATGGCTACCCAAGCTGAT	PLVX-IRES-β-catenin-puro
β-catenin-R (BamH I)	CGGGATCCCGTTACAGGTCAGTATCAAA	PLVX-IRES-β-catenin-puro
β-catenin-shRNA1-F	GATCCGGATGTGGATACCACCCAAGTCTCGAGACTTGGGTGGTATCCACATCCTTTTTG	β-catenin-shRNA1
β-catenin-shRNA1-R	AATTCAAAAAGGATGTGGATACCACCCAAGTCTCGAGACTTGGGTGGTATCCACATCCG	β-catenin-shRNA1
β-catenin-shRNA2-F	GATCCGCACAATCTTTCCCACCATCGCTCGAGCGATGGTGGGAAAGATTGTGCTTTTTG	β-catenin-shRNA2
β-catenin-shRNA2-R	AATTCAAAAAGCACAATCTTTCCCACCATCGCTCGAGCGATGGTGGGAAAGATTGTGCG	β-catenin-shRNA2
β-catenin-shRNA3-F	GATCCGCTTATGGCAATCAAGAAAGCCTCGAGGCTTTCTTGATTGCCATAAGCTTTTTG	β-catenin-shRNA3
β-catenin-shRNA3-R	AATTCAAAAAGCTTATGGCAATCAAGAAAGCCTCGAGGCTTTCTTGATTGCCATAAGCG	β-catenin-shRNA3

Note: Underline, underline stands for the restriction endonuclease enzyme cutting sites, Italic, italic stands for kozaka sequence, Bold, blod stands for Flag-tag sequence

ISSN: 1746-6148