Fig. 4

BPIV3 infection induced degradation of β-catenin via GSK3β (a) MDBK cells were infected or mock infected by BPIV3 at 1MOI for 36 h, and were subsequently harvested for western blot to detect GSK3β expression and β-catenin expression. Numbers below the image was GSK3β/β-actin ratios of band optical density values from Image J software. b Nucleus and cytoplasm were separated to detect the expression of β-catenin. MDBK cells were pretreated or not with LiCl of 20 mM for 24 h, and then were infected by BPIV3 at 1 MOI for 24 h. Subsequently, cells were harvested for nucleus and cytoplasm separation and western blot analysis. β-actin and Lamin B1 were used as an internal standard of cytoplasmic protein and nucleus protein, respectively. Numbers below the image was β-catenin/β-actin or β-catenin/Lamin B1 ratios of band optical density values