Skip to main content
Fig. 2 | BMC Veterinary Research

Fig. 2

From: Beta-catenin inhibits bovine parainfluenza virus type 3 replication via innate immunity pathway

Fig. 2

β-catenin inhibited BPIV3 replication in MDBK cells (a) The expression level of β-catenin protein in stable expressing cell lines was detected by western blot with anti-β-catenin antibody, anti-Flag antibody, and β-actin was used as an internal standard. Numbers below the image was β-catenin/β-actin ratios of band optical density values from Image J software. b The knockdown efficiency of β-catenin-shRNA recombinant vector in 293 T cells with anti-Flag antibody. c The screening about knockdown of β-catenin of MDBK cell lines, β-catenin-shRNA-2-1 was the effective silencing β-catenin cell lines, and named β-catenin-shRNA. Numbers below the image was β-catenin/β-actin ratios of band optical density values. d Effect of β-catenin silencing on BPIV3 replication. β-catenin knockdown cell lines and control cell lines were infected with BPIV3 (0.1 MOI) for 24 h, then the titers of BPIV3 infected cells were determined by TCID50 assay. The data were means with SD from four independent experiments (*, P < 0.05). e Effect of β-catenin overexpressing on BPIV3 replication. MDBK cells stably expressing β-catenin was infected BPIV3 (0.1 MOI) for 24 h, and cells were collected at 24 h and repeated freezing and thawing 3 times. Virus titers determined by the Reed-Muench endpoint method and expressed as log10 tissue culture infections dose 50 (TCID50) per milliliter. The results represent the average of three independent experiments (*, P < 0.05)

Back to article page