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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Fig. 1

Gating strategy of camel monocyte subsets. Camel leukocytes were separated by hypotonic lysis of erythrocytes and separated cells were labeled with monoclonal antibodies to CD14, CD172a and MHCII molecules and analyzed by flow cytometry. A) Mononuclear cells (PBMC, encircled region) were identified based on their forward (FSC) and side scatter (SSC) properties. B) In a SSC vs CD172a dot plot of gated PBMC, a region was set on CD172a-positive monocytes). C) Correlated dot plots of CD14 versus MHCII fluorescence after gating on CD172a-positive cells. Monocyte subsets mo I, II, and III were identified according to their CD14 and MHCII expression density (mo-I: CD14highMHCIIlow, mo-II: CD14highMHCIIhigh, mo-III: CD14lowMHCIIhigh)

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