Fig. 2

a Denaturing high-performance liquid chromotography (DHPLC) chromatograms: the peak profile of the case presented herein; the peak with a retention time of 1.53 min. (black arrow) indicates the presence of a heteroduplex caused by the presence of the mutation (c.1529C > T). the x-axis indicates DHPLC elution time in minutes and the y-axis the DNA concentration as measured by optical density at 260 nm in arbitrary units. A: b) Sanger sequencing chromatogram from the reverse primer of the renal sample and c) of the cutaneous samples, both indicating the presence of the primary (c.1523A > T; empty arrow) and secondary (c.1529C > T; plain arrowhead) mutations. The different heights of the peaks are evident. Likewise, the same pattern of mutations indicates the clonal origin of the primary lesion and distant metastasis