Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

Table 2 Values of histopathological analysis in control and methylene blue groups (MB5 and MB20)

Organ	Control	MB5	MB20
Duodenum	4 (2.8–6.8) ^a	1 (0.5–1.5) ^a	3 (2–4)
Jejunum	5.5 (4–6.5) ^a	2 (0.6–2.4) ^a,b	4 (4–6) ^b
Ileum	4 (3–4.5) ^a	1.25 (0.5–3.4) ^a,b	4 (3–5) ^b
Lung^c	2.5 (1–3)	2.5 (2–3)	2.5 (1–3)
Neutrophils infiltrate^d	2 (1.5–3)	2(2–2.5)	2 (1.8–2.8)
Liver^e	2 (1.5–3)	2 (1.6–2.5)	1.5 (1–3)
Kidney^f	2.5 (2–3)	2.75 (2.5–3)	2.5 (1.9–2.6)

Legend: Data are expressed as median (P₂₅–P₇₅)
^a. Significant difference (P < 0.05) between MB5 and control group
^b. Significant difference (p < 0.05) between MB5 and MB20
Scoring system followed for lung, neutrophils infiltrate, liver and kidney (more details in methods section):
^c0 (absence of lesions). 1 slight (focal lesions), 2 moderate (multifocal lesions), and 3 severe (diffuse lesions)
^d1 occasional (0–5 cells), 2 slight (6–15 cells), 3 moderate (16–30 cells), and 4 severe (> 30 cells)
^e0 (normal), 1 (slight), 2 (moderate); 3 (severe) to 4 (very severe)
^f0 (normal), 1 (slight; 0–50 vacuoles), 2 (moderate; 50–-100) and 3 (severe; > 100)

ISSN: 1746-6148