Effect of ovarian steroids on vascular endothelial growth factor a expression in bovine uterine endothelial cells during adenomyosis

Table 2 Oligonucleotide sequences used for real-time PCR

Gene	Oligonucleotide sequences	Product size (bp)	GeneBank
VEGFA	FWD 5′- CCATGAACTTTCTGCTCTCTTGG −3′	148	NM_174216.2
VEGFA	REV 5′- CTGCGCTGGTAGACATCCAT − 3′	148	NM_174216.2
VEGFR1	FWD 5′- ACGGTAGCCATCAGCAGTTC − 3′	188	NM_001191132.2
VEGFR1	REV 5′- GCTTTGCAGTGATACACGCC −3’	188	NM_001191132.2
VEGFR2	FWD 5′- CGCTCAACAGGATGGCAAAG −3’	85	NM_001110000.1
VEGFR2	REV 5′- AGGCAGAGAGAGTCCCGAAT −3’	85	NM_001110000.1
ACTB	FWD 5′-CCAAGGCCAACCGTGAGAAAAT-3’	256	K00622
ACTB	REV 5′- CCACATTCCGTGAGGATCTTCA-3’	256	K00622
RN18S	FWD 5′-AAGTCTTTGGGTTCCGGG-3’	365	AF176811
RN18S	REV 5′-GGACATCTAAGGGCATCACA −3’	365	AF176811
GAPDH	FWD 5′-CACCCTCAAGATTGTCAGCA-3’	103	BC102589
GAPDH	REV 5′-GGTCATAAGTCCCTCCACGA-3’	103	BC102589

VEGFA vascular endotelial growth factor A, VEGFR1 vascular endotelial growth factor receptor 1, VEGFR2 vascular endotelial growth factor receptor 2, ACTB beta actin, RN18S 18S ribosomal RNA, GAPDH glyceraldehyde-3-phosphate dehydrogenase

ISSN: 1746-6148