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Table 3 Comparison of Multiplex sodA-PCR and 16S rRNA sequencing in identification of Enterococcus species in racing pigeons

From: Prevalence, antibiotic susceptibility and virulence factors of Enterococcus species in racing pigeons (Columba livia f. domestica)

 

Enterococcus spp.

16S Sequencing

Total (n)

E. columbae

E. hirae

E. faecium

E. faecalis

E. gallinarum

E. mundtii

E. casseliflavus

E. cecorum

E. durans

Multiplex PCR (sodA)

E. columbae

50

0

0

0

0

0

0

0

0

50

E. hirae

2

28

0

0

0

0

0

0

0

30

E. faecium

1

0

16

0

0

0

0

0

0

17

E. faecalis

0

0

0

17

0

0

0

0

0

17

E. gallinarum

0

0

0

0

13

0

0

0

0

13

E. mundtii

1

0

0

0

0

6

0

0

0

7

E. casseliflavus

0

0

0

0

0

0

5

0

0

5

E. cecorum

0

0

0

0

0

0

0

3

0

3

E. durans

1

0

0

0

0

0

0

0

2

3

Total (n)

55

28

16

17

13

6

5

3

2

145

  1. Cohen’s kappa = 0.956 (CI 95%: 0.919, 0.993) – almost perfect agreement
  2. Green indicates agreement in species identification between sequencing and PCR; yellow indicates a lack of agreement