Table 4 Primers used for 16S rRNA and 12 virulence genes
Genes | Primer sequence (5′-3′) | Product size (bp) | Reference |
|---|---|---|---|
16S rRNA | F: ccgaattcgtcgacaacagagtttgatcctggctcag | 1549 | 23 |
R: cccgggatccaagcttaaggaggtgatccagcc | |||
ptfA | F: tgtggaattcagcattttagtgtgtc | 488 | 24 |
R: tcatgaattcttatgcgcaaaatcctgctgg | |||
tadD | F: tctacccattctcagcaaggc | 416 | 24 |
R: atcatttcgggcattcacc | |||
pfhA | F: ttcagagggatcaatcttcg | 286 | 24 |
R: aactccagttggtttgtcg | |||
toxA | F: cttagatgagcgacaagg | 864 | 24 |
R: gaatgccacacctctatag | |||
fur | F: gtttaccgtgtattagacca | 244 | 24 |
R: cattactacatttgccatac | |||
tbpA | F: ttggttggaaacggtaaagc | 729 | 15 |
R: taacgtgtacggaaaagccc | |||
hgbB | F: accgcgttggaattatgattg | 789 | 15 |
R: cattgagtacggcttgacat | |||
nanB | F: cattgcacctaacacctct | 555 | 24 |
R: ggacactgattgccctgaa | |||
pmHAS | F: tcaatgtttgcgatagtccgttag | 430 | 24 |
R: tggcgaatgatcggtgataga | |||
ompA | F: cgcatagcactcaagtttctcc | 201 | 24 |
R: cataaacagattgaccgaaacg | |||
ompH | F: cgcgtatgaaggtttaggt | 438 | 24 |
R: tttagattgtgcgtagtcaac | |||
oma87 | F: ggcagcgagcaacagataacg | 838 | 24 |
R: tgttcgtcaaatgtcgggtga |