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Table 1 List of Potential Risk Factors (PRFs) selected for screening process

From: The British E. coli O157 in cattle study (BECS): factors associated with the occurrence of E. coli O157 from contemporaneous cross-sectional surveys

PRF

Explanation

Comments

total cattle

Total number of cattle on the farm on sampling day

Continuous

management type

Cattle management type the respondent indicated as primary system on the farm

Categorical, 4 levels; Dairy (used as baseline level in the analysis); Other, Suckler Beef; Specialist Finisher.

AHDa

Animal Health Division – a non-current geographical designation in Scotland

Categorical, 6 levels

cattle 12–30 months

Total number of cattle on the farm aged between 12 and 30 months on sampling day

Continuous

cattle less than 1 year

Total number of cattle on the farm aged under 1 year on sampling day

Continuous

group size

Total number of cattle in the sample group

Continuous

oldest in group

Age (months) of the oldest sample group animal

Continuous

youngest in group

Age (months) of the youngest sample group animal

Continuous

season

Calendar year divided into three-month periods with March–May = spring, etc.

Categorical, 4 levels: Autumn (used as baseline level in the analysis); Winter, Spring; Summer

housed

Whether the sample group was completely housed or had access to grazing

Dichotomous; baseline “grazing”

feed changed

Whether or not the sample group’s feed had changed in the 2 weeks before sampling

Dichotomous; baseline “no”

location changed

Whether or not the sample group were moved in the 2 weeks before sampling

Dichotomous; baseline “no”

cattle brought on (CBO)

Whether or not cattle were moved on to the farm in the year before sampling

Dichotomous; baseline “no”

breeding females brought on (BFBO)

Whether or not breeding female cattle were moved onto the farm in the year before sampling.

Dichotomous; baseline “no”

fatteners brought on (FBO)

Whether or not fattening cattle had been moved onto the farm in the year before sampling.

Dichotomous; baseline “no”

livestock on farm not owned by farmer

Whether or not there were livestock of any species present on the day of sampling, which were not owned by the respondent (e.g. over-wintering sheep)

Dichotomous; baseline “no”

bought other livestock

Whether or not any livestock (not cattle) had been purchased in the year before sampling

Dichotomous; baseline “no”

cattle elsewhere

Whether or not employed farm workers caring for cattle on the sampled farm could also come into contact with cattle through work or family/friends elsewhere.

Dichotomous; NotApp answers categorised as “no”; baseline “no”

non mains water

Whether or not sampled cattle had access to non-mains water

Dichotomous; baseline “no”

organic

Whether or not routine farm practice was to spread cattle manure or slurry on grazing/silage ground

Dichotomous; NotApp answers categorised as “no”; baseline “no”

wild geese

Whether or not flocks of 100 or more geese had been seen on fields to which cattle have access

Dichotomous; baseline “no”

gulls

Whether or not flocks of 100 of more gulls had been seen on fields to which cattle have access

Dichotomous; baseline “no”

ewes

Whether or not there were breeding ewes present on the farm on sampling day

Dichotomous; baseline “no”

surveyb

Whether or not the survey was conducted in Scotland or in England & Wales

Dichotomous; baseline “no”

stx statusc

Indicating which Shiga toxins were present in faecal pat samples collected from a positive farm

Categorical, 3 levels: stx1&stx2_neg (baseline); stx1neg_stx2pos; stx1&stx2pos

percent posc

The percentage of all faecal pat samples collected from a positive farm, which tested individually positive for E. coli O157

Continuous

  1. The majority of PRFs on this list were tested for all three outcomes for interest
  2. NotApp Not applicable, PRF, potential risk factor; stx shiga toxin
  3. a PRF only used for the analysis of Scotland data
  4. b PRF only used for the analysis of the combined data sets from both surveys
  5. c PRF only used for the analysis of whether or not a super-shedder sample was present in samples from a positive farm (Outcome 3)