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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Plant-produced chimeric virus-like particles - a new generation vaccine against African horse sickness

Fig. 3

Immunoblot detection of AHSV-1 and/or AHSV-7 capsid proteins in 55–50% sucrose fractions. Precision Plus Protein™ Western C™ standard (Bio-Rad) is the marker (M) and the relevant sizes are indicated. Fractions of the AHSV-1 CLP gradient, the AHSV-1 VLP gradient, chimeric AHSV-1/AHSV-7 VLP gradient and double chimeric (dc) AHSV-1/AHSV-7 VLP gradient are present in lanes 2–3, 4–5, 6–7 and 8–9, respectively. Indicated above the lanes are the sucrose concentrations of the fractions. Immunoblotting was performed with a guinea pig anti-AHSV-7 serum. Arrows indicate the position of the AHSV-7 VP2 (123,6KDa), AHSV-1 VP3 (103.2KDa), AHSV-1 VP5 or AHSV-7 VP5 (56.6KDa) and AHSV-1 VP7 (37.8KDa) proteins on the immunoblot membrane

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BMC Veterinary Research

ISSN: 1746-6148

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