Table 2 The primer sequences used for the studied cytokines and reference genes

Interleukin 1 alpha

IL-1α

F:TGAACGACGCCCTCAATCAA

R: CTTGCCATGTGCACCAGTTT

423-442

789-761

358

D63350.1

designed

Interleukin 1 beta

IL-1β

F: GCAGCTGGAGGAAGTAGACC

r: TGGCTTTCTTTAGGGAGAGAGG

585-604

816-795

232

D63351.1

designed

Interleukin 2

IL-2

F: ATGTACCAGATACCACTCTTGTCTT

R: TCAAGTCATTGTTGAGTAGAT

22–46

489–469

468

AY603404.1

(9)

Interleukin 4

IL-4

F:TAGCTTCTCCTGATAAACTA

R: ATGAGTTATAAATATATAAATA

1–20

535–514

535

U34273.1

(9)

Interleukin 6

IL-6

F: TCTTCACAAGCGCCTTCAGT

R: CTGCTTGGGGTGGTGTCATT

11-30

131-112

121

D86569.1

designed

Interleukin 10

IL-10

F: CGGCGCTGTCATCGTTTT

R: TCTTGGAGCATATTGAAGACTCTCTTC

364-381

446-420

83

DQ837159.1

(11)

Interleukin 12

IL-12

F: CACCAAAGATAAAACCAGCACAGT

R: GTCTTTCCAGAAGCCAGACAATG

180-203

305-283

126

AY603407.1

(11)

Interleukin 16

IL-16

F: AAAAGACCTCTGCGGGACTG

R: TCAGGCAACGCCTTGATGAT

101-120

314-295

214

AF481158.1

designed

Interleukin 18

IL-18

F: TCCTAAGAAGCTATTGAGCACAGGC

R: ATTTTAATATCTAGTCTGGTTTTG

9-33

628-604

611

AY605263.1

(33)

Interferon alpha

IFN-α

F: CACCTTCCAGCTCTTCAGCA

R: GTCAGTGAGCTGCTGATCCA

258-277

354-335

97

FJ959074.1

designed

Interferon beta

IFN-β

F: ACAGCAGTTCCGGAAGGAAG

R: TCGGTCGTGTCTCCCATAGT

204-223

416-397

213

JX458085.1

designed

Interferon gamma

IFN-γ

F: TAGCTAAGGGTGGGCCTCTTTTCTCA

R:TGCAGGCAGGAGAACCATTACATTGA

189–214

573-548

385

AY603405.1

(9)

Tumor NecrosisFactoralpha

TNF-α

F: AGAAGGGAGATCGCCTCAGT

R: AGAAGGGGATGAGGAGGGTC

488-507

659-640

172

X14828.1

designed

Cyclophilin A

PPIA

F:GGATTTATGTGCCAGGGTGGTGA

R: CAAGATGCCAGGACCTGTATG

186-208

305-285

120

AY 247029.1

(36)

Zeta polypeptide

YWHAZ

F:AGACGGAAGGTGCTGAGAAA

R:CGTTGGGGATCAAGAACTTT

290-309

412-393

122

BC102382.1

(36)