A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs

Table 1 B. gibsoni-positive dog blood samples included in this study

No.	Age (yr)	Sex	Breed	PCV^a (%)	Test results		Parasites/μL of blood^b
No.	Age (yr)	Sex	Breed	PCV^a (%)	QubeMDx	Real-time PCR	Parasites/μL of blood^b
1	8	M	Mixed	n/a	+	+	94
2	7	M	n/a	32.3	+	+	98
3	12	F	Mixed	28.8	+	+	103
4	13	M	Mixed	21.3	+	+	188
5	4.3	F	Mixed	50.5	+	+	249
6	1.7	M	Miniature Schnauzer	23.6	+	+	261
7	14	F	Mixed	27.3	+	+	422
8	9	F	Mixed	31.8	+	+	798
9	2	F	Mixed	n/a	+	+	1030
10	5	M	Poodle	37.8	+	+	1150
11	8	M	Mixed	48.7	+	+	1290
12	6	F	Mixed	n/a	+	+	1360
13	5	M	Poodle	37.7	+	+	1870
14	6	F	Mixed	n/a	+	+	2180
15	5	F	Mixed	n/a	+	+	3610
16	9	M	Labrador Retriever	25.9	+	+	3840
17	4	F	Mixed	43.9	+	+	5410
18	6	M	Poodle	47.3	+	+	8140
19	3	M	Poodle	14.6	+	+	8140
20	2	F	Mixed	51.2	+	+	8760
21	0.2	F	Mixed	30.3	+	+	10,100
22	12	F	Miniature Schnauzer	16.7	+	+	10,800
23	9	F	Mixed	n/a	+	+	13,700
24	6	M	Corgi	10.5	+	+	14,200
25	2	M	Pomeranian	40.6	+	+	28,800
26	12	M	Dachshund	16	+	+	30,400
27	0.5	M	Bichon Frisé	18.6	+	+	149,000
28	10	M	Maltese	14.6	+	+	398,000

^aPacked cell volume
^bParasites was determined by real-time PCR and calculated from the standard curve constructed with the plasmid DNA according to Matsuu et al., 2005 [15]

ISSN: 1746-6148