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Table 3 Detailed results from all the experiments performed to determine the vector copy number (VCN)/cell after transduction with the various lentiviral vectors

From: Development of a CRISPR/Cas9 system against ruminant animal brucellosis

  VCN/cell
D1 post transduction
VCN/cell
D4 post transduction
Brucella untransducedExp 10.050.14
Exp 20.020.05
Exp 30.120.04
Exp 40.000.01
 Average0.050.06
SD0.050.06
Brucella + GFPExp 15.156.21
Exp 26.762.48
Exp 322.4714.02
Exp 423.937.80
 Average14.587.63
SD10.004.81
Brucella + VirB10Exp 10.800.30
Exp 215.2411.94
Exp 31.840.13
Exp 414.2410.39
 Average8.035.69
SD7.776.35
Brucella + RpolAExp 111.073.94
Exp 24.1911.89
Exp 39.914.89
Exp 422.858.52
 Average12.017.31
SD7.833.64
  1. The standard curves produced by quantitative Real-Time PCR were used to extrapolate the absolute number of vector and endogenous ovPrp copies per reaction. VCN/cell was ultimately calculated by normalizing the absolute number of vector copies to the ovPrp copies. Untransduced, Brucella-infected cells served as a control