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Table 3 Detailed results from all the experiments performed to determine the vector copy number (VCN)/cell after transduction with the various lentiviral vectors

From: Development of a CRISPR/Cas9 system against ruminant animal brucellosis

  

VCN/cell

D1 post transduction

VCN/cell

D4 post transduction

Brucella untransduced

Exp 1

0.05

0.14

Exp 2

0.02

0.05

Exp 3

0.12

0.04

Exp 4

0.00

0.01

 

Average

0.05

0.06

SD

0.05

0.06

Brucella + GFP

Exp 1

5.15

6.21

Exp 2

6.76

2.48

Exp 3

22.47

14.02

Exp 4

23.93

7.80

 

Average

14.58

7.63

SD

10.00

4.81

Brucella + VirB10

Exp 1

0.80

0.30

Exp 2

15.24

11.94

Exp 3

1.84

0.13

Exp 4

14.24

10.39

 

Average

8.03

5.69

SD

7.77

6.35

Brucella + RpolA

Exp 1

11.07

3.94

Exp 2

4.19

11.89

Exp 3

9.91

4.89

Exp 4

22.85

8.52

 

Average

12.01

7.31

SD

7.83

3.64

  1. The standard curves produced by quantitative Real-Time PCR were used to extrapolate the absolute number of vector and endogenous ovPrp copies per reaction. VCN/cell was ultimately calculated by normalizing the absolute number of vector copies to the ovPrp copies. Untransduced, Brucella-infected cells served as a control