Table 2 Detailed results from all the experiments performed to determine the number of incorporated brucellae after transduction with the various lentiviral vectors (CFU assay from 500 infected and lysed macrophages seeded in blood agar dishes and from 50,000 infected macrophages analyzed by Real-Time PCR)
From: Development of a CRISPR/Cas9 system against ruminant animal brucellosis
| Â | D1 post transduction | D4 post transduction | |||
|---|---|---|---|---|---|
Total number of colonies (agar) | Brucellae/cell (Real-Time PCR) | Total number of colonies (agar) | Brucellae/cell (Real-Time PCR) | ||
Brucella untransduced | Exp 1 | 55 | 412.69 | 35 | 210.09 |
Exp 2 | 64 | 488.82 | 36 | 483.57 | |
Exp 3 | 51 | 513.84 | 40 | 211.50 | |
Exp 4 | 60 | 374.00 | 43 | 201.51 | |
| Â | Average | 57.5 | 447.34 | 38.5 | 276.67 |
SD | 5.68 | 65.12 | 3.69 | 138.01 | |
Brucella + GFP | Exp 1 | 66 | 243.54 | 32 | 141.28 |
Exp 2 | 57 | 371.39 | 33 | 195.71 | |
Exp 3 | 53 | 472.10 | 40 | 198.36 | |
Exp 4 | 50 | 532.71 | 39 | 204.05 | |
| Â | Average | 56.5 | 404.94 | 36 | 184.85 |
SD | 6.95 | 126.51 | 4.08 | 29.26 | |
Brucella + VirB10 | Exp 1 | 30 | 259.82 | 21 | 181.39 |
Exp 2 | 32 | Undetermined | 28 | 133.04 | |
Exp 3 | 31 | 318.92 | 30 | 156.65 | |
Exp 4 | 37 | 228.00 | 23 | 125.00 | |
| Â | Average | 32.5 | 268.91 | 25.5 | 149.02 |
SD | 3.1 | 46.14 | 4.2 | 25.42 | |
Brucella + RpolA | Exp 1 | 22 | Undetermined | 15 | 161.01 |
Exp 2 | 26 | 221.09 | 18 | 139.49 | |
Exp 3 | 22 | 161.78 | 20 | 65.47 | |
Exp 4 | 23 | 107.84 | 11 | 60.42 | |
| Â | Average | 23.25 | 163.57 | 16 | 106.6 |
SD | 1.89 | 56.65 | 3.91 | 51.21 | |