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Table 1 The absolute number of live macrophages per well during the experiment, measured by trypan blue exclusion

From: Development of a CRISPR/Cas9 system against ruminant animal brucellosis

  D1 post transductionD4 post transduction
Brucella untransducedExp 175,110110,000
Exp 272,125101,975
Exp 370,000102,500
Exp 484,323100,075
 Average75,389.5103,637.5
SD6313.74367.7
Brucella + GFPExp 174,000105,000
Exp 273,500101,700
Exp 368,00098,000
Exp 478,575105,000
 Average73,518.7102,425
SD4330.23335
Brucella + VirB10Exp 185,00099,700
Exp 273,000110,000
Exp 381,500103,400
Exp 476,500108,575
 Average79,000105,418.7
SD 5307.24751.4
Brucella + RpolAExp 176,580106,000
Exp 280,475107,800
Exp 390,000110,350
Exp 476,00099,900
 Average80,763.7106,012.5
SD6470.14448.6
  1. All experimental conditions were initiated by seeding 50,000 macrophages per well. Untransduced, Brucella-infected cells served as a control