Skip to main content

Table 1 The absolute number of live macrophages per well during the experiment, measured by trypan blue exclusion

From: Development of a CRISPR/Cas9 system against ruminant animal brucellosis

  

D1 post transduction

D4 post transduction

Brucella untransduced

Exp 1

75,110

110,000

Exp 2

72,125

101,975

Exp 3

70,000

102,500

Exp 4

84,323

100,075

 

Average

75,389.5

103,637.5

SD

6313.7

4367.7

Brucella + GFP

Exp 1

74,000

105,000

Exp 2

73,500

101,700

Exp 3

68,000

98,000

Exp 4

78,575

105,000

 

Average

73,518.7

102,425

SD

4330.2

3335

Brucella + VirB10

Exp 1

85,000

99,700

Exp 2

73,000

110,000

Exp 3

81,500

103,400

Exp 4

76,500

108,575

 

Average

79,000

105,418.7

SD

5307.2

4751.4

Brucella + RpolA

Exp 1

76,580

106,000

Exp 2

80,475

107,800

Exp 3

90,000

110,350

Exp 4

76,000

99,900

 

Average

80,763.7

106,012.5

SD

6470.1

4448.6

  1. All experimental conditions were initiated by seeding 50,000 macrophages per well. Untransduced, Brucella-infected cells served as a control