Table 1 The summary of the result of serological assays in each group. (+): positive; (−): negative; NE: non-examination; C-N: conventional PEDV naïve pigs; C-I: experimental PEDV inoculated conventional pigs; SPF-N: specific pathogen free PEDV naïve pigs; SPF-I: experimental PEDV inoculated SPF pigs; Sow-F: PEDV-feedback sows. NE: non-examined
Group | No. of pigs | Commercial N-based ELISA | Full-length S-based ELISA | S1–501-based ELISA | |||
|---|---|---|---|---|---|---|---|
+ | – | + | – | + | – | ||
C-N | 55 | 0 | 55 | 5 | 50 | 0 | 55 |
144 | NE | NE | 6 | 138 | 2 | 142 | |
C-I | 23 | 8 | 15 | 23 | 0 | 23 | 0 |
27 | NE | NE | 25 | 2 | 26 | 1 | |
SPF-N | 14 | 0 | 14 | 0 | 14 | 0 | 14 |
SPF-I | 14 | 11 | 3 | 14 | 0 | 14 | 0 |
Sow-F | 26 | 4 | 22 | 26 | 0 | 26 | 0 |
Sensitivity | 37% | 97.8% | 98.9% | ||||
Specificity | 100% | 94% | 99.1% | ||||
Kappa value | 0.37 | 0.90 | 0.97 | ||||