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Fig. 7 | BMC Veterinary Research

Fig. 7

From: Development and comparison of enzyme-linked immunosorbent assays based on recombinant trimeric full-length and truncated spike proteins for detecting antibodies against porcine epidemic diarrhea virus

Fig. 7

Expression and characterization of G1b PEDV S protein by ICC staining and western blotting. a ICC staining of G1b PEDV S-expressing HEK cells using anti-V5 tag antibody. The cells were fixed on the plates using acetone, probed using 1000-fold diluted anti-V5 tag antibody, and detected using anti-mouse IgG antibody conjugated with HRP. The coloration procedure was carried out using the DAB system. b The negative control of the ICC staining of non-transfected HEK cells. The cells were probed with anti-V5 tag antibody following by the anti-mouse IgG antibody conjugated with HRP to distinguish the non-specific signals. c The molecular weight of the purified G1b PEDV S protein (Lane 1) was estimated by western blotting. The purified G1b PEDV S protein was denatured in NuPAGE® LDS sample buffer containing the NuPAGE® reducing agent and boiled at 95 °C for 5 min, then, separated by SDS-PAGE, transferred onto the PVDF membrane, and stained with anti-V5 tag antibody. The HEK cell lysate was used as the negative control (Lane 2). The ladder is shown in kilodalton (kDa)

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