Skip to main content

Table 3 Primers and probes oligonucleotides for real-time-PCR assays

From: Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum

Targeted genes or sequences Primers-Probe name Sequence 5′ – 3′ Origin
IS6110 TR IS6110 F GGT AGC AGA CCT CAC CTA TGT GT LNR, ANSES
  TR IS6110 R AGG CGT CGG TGA CAA AGG LNR, ANSES
TR IS6110 P (FAM)-CAC GTA GGC GAA CCC-(MGB-NFQ) LNR, ANSES
IS1081 TR IS1081 F CCG CCA CCG TGA TTT CGA LNR, ANSES
  TR IS1081 R GCC AGT CCG GGA AAT AGC T LNR, ANSES
TR IS1081 P (FAM)-CCG CAA CCA TCG ACG TC-(MGB-NFQ) LNR, ANSES
IS1245 TR IS1245 F GCC GCC GAA ACG ATC TAC LNR, ANSES
  TR IS1245 R TGA CCC GGT GCG CAG CTT LNR, ANSES
TR IS1245 P (FAM)-TCG CGT CCG CGC ACG CTG TCC A-(BHQ1) LNR, ANSES
Hsp65 F MSP GCC AAG GAG GTC GAG ACC AA LNR, ANSES
  R MSP CTC CTC GAC GGT GAT GAC  
P MSP (FAM)-ACC TTG TCC ATC GCC TCG GCG AT-(BHQ1)
RD1 flanking region (BCG) RD1 F TAC GCT CGC GTT CGT GGT LNR, ANSES
  RD1 R GAT GAG TAT TAC CAG GCC GAC  
S RD1 (FAM)-TCC GGG CGG CTG GGT GAT GTG -(BHQ1)
IS1561’ TR IS1561’ F GAT CCA GGC CGA GAG AAT CTG [44]
  TR IS1561’ R GGA CAA AAG CTT CGC CAA AA  
TR IS1561’ P (FAM)- ACG GCG TTG ATC CGA TTC CGC-(BHQ1)
Rv1510 Rv1510 F CCA CGA CTA TGA CTA GGA CAG CAA [45]
  Rv1510 R AAG AAC TAT CAA TCG GGC AAG ATC  
Rv1510 S (FAM)- ACC AGT GAG GAA ACC-(MGB-NFQ)