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Table 3 Primers and probes oligonucleotides for real-time-PCR assays

From: Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum

Targeted genes or sequencesPrimers-Probe nameSequence 5′ – 3′Origin
IS6110TR IS6110 FGGT AGC AGA CCT CAC CTA TGT GTLNR, ANSES
 TR IS6110 RAGG CGT CGG TGA CAA AGGLNR, ANSES
TR IS6110 P(FAM)-CAC GTA GGC GAA CCC-(MGB-NFQ)LNR, ANSES
IS1081TR IS1081 FCCG CCA CCG TGA TTT CGALNR, ANSES
 TR IS1081 RGCC AGT CCG GGA AAT AGC TLNR, ANSES
TR IS1081 P(FAM)-CCG CAA CCA TCG ACG TC-(MGB-NFQ)LNR, ANSES
IS1245TR IS1245 FGCC GCC GAA ACG ATC TACLNR, ANSES
 TR IS1245 RTGA CCC GGT GCG CAG CTTLNR, ANSES
TR IS1245 P(FAM)-TCG CGT CCG CGC ACG CTG TCC A-(BHQ1)LNR, ANSES
Hsp65F MSPGCC AAG GAG GTC GAG ACC AALNR, ANSES
 R MSPCTC CTC GAC GGT GAT GAC 
P MSP(FAM)-ACC TTG TCC ATC GCC TCG GCG AT-(BHQ1)
RD1 flanking region (BCG)RD1 FTAC GCT CGC GTT CGT GGTLNR, ANSES
 RD1 RGAT GAG TAT TAC CAG GCC GAC 
S RD1(FAM)-TCC GGG CGG CTG GGT GAT GTG -(BHQ1)
IS1561’TR IS1561’ FGAT CCA GGC CGA GAG AAT CTG[44]
 TR IS1561’ RGGA CAA AAG CTT CGC CAA AA 
TR IS1561’ P(FAM)- ACG GCG TTG ATC CGA TTC CGC-(BHQ1)
Rv1510Rv1510 FCCA CGA CTA TGA CTA GGA CAG CAA[45]
 Rv1510 RAAG AAC TAT CAA TCG GGC AAG ATC 
Rv1510 S(FAM)- ACC AGT GAG GAA ACC-(MGB-NFQ)