Table 3 Primers and probes oligonucleotides for real-time-PCR assays
Targeted genes or sequences | Primers-Probe name | Sequence 5′ – 3′ | Origin |
|---|---|---|---|
IS6110 | TR IS6110 F | GGT AGC AGA CCT CAC CTA TGT GT | LNR, ANSES |
TR IS6110 R | AGG CGT CGG TGA CAA AGG | LNR, ANSES | |
TR IS6110 P | (FAM)-CAC GTA GGC GAA CCC-(MGB-NFQ) | LNR, ANSES | |
IS1081 | TR IS1081 F | CCG CCA CCG TGA TTT CGA | LNR, ANSES |
TR IS1081 R | GCC AGT CCG GGA AAT AGC T | LNR, ANSES | |
TR IS1081 P | (FAM)-CCG CAA CCA TCG ACG TC-(MGB-NFQ) | LNR, ANSES | |
IS1245 | TR IS1245 F | GCC GCC GAA ACG ATC TAC | LNR, ANSES |
TR IS1245 R | TGA CCC GGT GCG CAG CTT | LNR, ANSES | |
TR IS1245 P | (FAM)-TCG CGT CCG CGC ACG CTG TCC A-(BHQ1) | LNR, ANSES | |
Hsp65 | F MSP | GCC AAG GAG GTC GAG ACC AA | LNR, ANSES |
R MSP | CTC CTC GAC GGT GAT GAC | ||
P MSP | (FAM)-ACC TTG TCC ATC GCC TCG GCG AT-(BHQ1) | ||
RD1 flanking region (BCG) | RD1 F | TAC GCT CGC GTT CGT GGT | LNR, ANSES |
RD1 R | GAT GAG TAT TAC CAG GCC GAC | ||
S RD1 | (FAM)-TCC GGG CGG CTG GGT GAT GTG -(BHQ1) | ||
IS1561’ | TR IS1561’ F | GAT CCA GGC CGA GAG AAT CTG | [44] |
TR IS1561’ R | GGA CAA AAG CTT CGC CAA AA | ||
TR IS1561’ P | (FAM)- ACG GCG TTG ATC CGA TTC CGC-(BHQ1) | ||
Rv1510 | Rv1510 F | CCA CGA CTA TGA CTA GGA CAG CAA | [45] |
Rv1510 R | AAG AAC TAT CAA TCG GGC AAG ATC | ||
Rv1510 S | (FAM)- ACC AGT GAG GAA ACC-(MGB-NFQ) |