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Table 1 Summary of vaccination protocols and tests (bacteriology, histology and immunology)

From: Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum

 Groups
Group AGroup BGroup CGroup D
Number of animals6644
Study12
Vaccine deliveryIntellicap capsuleOropharyngealOropharyngealOropharyngeal
Lipid addedNoHPOCocoa Butter
Mucosal targetIntestineOralOralOral
Volume of vaccineIleum300 μl   
Tonsils 100 μl200 μl200 μl
Sub-lingual100 μl  
Vaccine dose (CFU)5.67 × 1076.73 × 107
Culture sampleSectionSectionCompleteComplete
Inoculum volume per 7H11 agar plate400 μl100 μl
Volume of saline added per sample3, 5 or 10 ml saline to 0–0.5 g, 0.5–1 g and > 1 g tissues, respectively10 ml saline
RT-PCR primersIS6110, IS1081, IS1561’, hsp65, RD1 flanking regionIS6110, IS1081, IS1561’, RV1510
Tissues pooledNoneLeft and right tonsils (pool 1), retro-pharyngeal (pool 2) and mandibular (pool 3) lymph nodes
HistologyYesNo
Tracheal section washYesNo
Environmental faecal sampleYesNo
IGRAYesYes
ELISPOTYesNo