Groups | |||||
---|---|---|---|---|---|
Group A | Group B | Group C | Group D | ||
Number of animals | 6 | 6 | 4 | 4 | |
Study | 1 | 2 | |||
Vaccine delivery | Intellicap capsule | Oropharyngeal | Oropharyngeal | Oropharyngeal | |
Lipid added | No | HPO | Cocoa Butter | ||
Mucosal target | Intestine | Oral | Oral | Oral | |
Volume of vaccine | Ileum | 300 μl | |||
Tonsils | 100 μl | 200 μl | 200 μl | ||
Sub-lingual | 100 μl | ||||
Vaccine dose (CFU) | 5.67 × 107 | 6.73 × 107 | |||
Culture sample | Section | Section | Complete | Complete | |
Inoculum volume per 7H11 agar plate | 400 μl | 100 μl | |||
Volume of saline added per sample | 3, 5 or 10 ml saline to 0–0.5 g, 0.5–1 g and > 1 g tissues, respectively | 10 ml saline | |||
RT-PCR primers | IS6110, IS1081, IS1561’, hsp65, RD1 flanking region | IS6110, IS1081, IS1561’, RV1510 | |||
Tissues pooled | None | Left and right tonsils (pool 1), retro-pharyngeal (pool 2) and mandibular (pool 3) lymph nodes | |||
Histology | Yes | No | |||
Tracheal section wash | Yes | No | |||
Environmental faecal sample | Yes | No | |||
IGRA | Yes | Yes | |||
ELISPOT | Yes | No |