Number of animals | 6 | 6 | 4 | 4 |
Study | 1 | 2 |
Vaccine delivery | Intellicap capsule | Oropharyngeal | Oropharyngeal | Oropharyngeal |
Lipid added | No | HPO | Cocoa Butter |
Mucosal target | Intestine | Oral | Oral | Oral |
Volume of vaccine | Ileum | 300 μl | | | |
Tonsils | | 100 μl | 200 μl | 200 μl |
Sub-lingual | 100 μl | | |
Vaccine dose (CFU) | 5.67 × 107 | 6.73 × 107 |
Culture sample | Section | Section | Complete | Complete |
Inoculum volume per 7H11 agar plate | 400 μl | 100 μl |
Volume of saline added per sample | 3, 5 or 10 ml saline to 0–0.5 g, 0.5–1 g and > 1 g tissues, respectively | 10 ml saline |
RT-PCR primers | IS6110, IS1081, IS1561’, hsp65, RD1 flanking region | IS6110, IS1081, IS1561’, RV1510 |
Tissues pooled | None | Left and right tonsils (pool 1), retro-pharyngeal (pool 2) and mandibular (pool 3) lymph nodes |
Histology | Yes | No |
Tracheal section wash | Yes | No |
Environmental faecal sample | Yes | No |
IGRA | Yes | Yes |
ELISPOT | Yes | No |