Number of animals
|
6
|
6
|
4
|
4
|
Study
|
1
|
2
|
Vaccine delivery
|
Intellicap capsule
|
Oropharyngeal
|
Oropharyngeal
|
Oropharyngeal
|
Lipid added
|
No
|
HPO
|
Cocoa Butter
|
Mucosal target
|
Intestine
|
Oral
|
Oral
|
Oral
|
Volume of vaccine
|
Ileum
|
300 μl
| | | |
Tonsils
| |
100 μl
|
200 μl
|
200 μl
|
Sub-lingual
|
100 μl
| | |
Vaccine dose (CFU)
|
5.67 × 107
|
6.73 × 107
|
Culture sample
|
Section
|
Section
|
Complete
|
Complete
|
Inoculum volume per 7H11 agar plate
|
400 μl
|
100 μl
|
Volume of saline added per sample
|
3, 5 or 10 ml saline to 0–0.5 g, 0.5–1 g and > 1 g tissues, respectively
|
10 ml saline
|
RT-PCR primers
|
IS6110, IS1081, IS1561’, hsp65, RD1 flanking region
|
IS6110, IS1081, IS1561’, RV1510
|
Tissues pooled
|
None
|
Left and right tonsils (pool 1), retro-pharyngeal (pool 2) and mandibular (pool 3) lymph nodes
|
Histology
|
Yes
|
No
|
Tracheal section wash
|
Yes
|
No
|
Environmental faecal sample
|
Yes
|
No
|
IGRA
|
Yes
|
Yes
|
ELISPOT
|
Yes
|
No
|