Table 1 Summary of ELISA kit characteristics

Coating antigen

rmCL1

f2

E/S protein

MM3

Antigen source

Recombinant [17]

Purified from natural F. hepatica E/S products [34]

Purified from supernatant of F. hepatica incubation media [35]

Purified from supernatant of F. hepatica incubation media [36]

Sample types

Serum, milk, BTM

Serum, milk, BTM

Serum, milk, BTM, meat juice

Serum, milk, BTM

Serum sample dilution

1/20

1/20

1/100

1/100

Milk sample dilution

Not required

1/20

Not required

1/4

Total incubation

70 min

110 min

150 min

130 min

Optical density

450 nm

450 nm

405 nm

450 nm

Serum Positive Cut-off

≥ 15S/P%

> 30S/P%

≥ 0.4ODR^a

≥ 10S/P%