Table 1 Relative gene expression in the BALF of 7 horses with mild equine asthma compared to healthy controls on day 0. The normalization factor (calculated from multiple reference genes) has a value of 1, and genes of interest are either up-regulated or down-regulated, in association with lower airway inflammation. * indicates a significant difference (p < 0.05)
Gene | Expression | Regulation | Standard Error | 95% C.I. | P value |
|---|---|---|---|---|---|
IL-1β | 1.15 | DOWN | 0.07–7.25 | 0.01–18.51 | 0.79 |
IL-4 | 1.96 | UP | 0.31–13.03 | 0.09–48.31 | 0.12 |
IL-5 | 1.29 | DOWN | 0.08–14.83 | 0.01–40.83 | 0.69 |
IL-6 | 3.97 | DOWN | 0.01–4.80 | 0.00–66.38 | 0.09 |
IL-8 | 1.96 | DOWN | 0.11–2.67 | 0.02–8.00 | 0.08 |
IL-10 | 2.56 | DOWN | 0.06–2.73 | 0.01–8.37 | 0.049* |
IL-12 | 1.14 | DOWN | 0.18–3.74 | 0.04–9.59 | 0.72 |
IL-17 | 4.95 | UP | 0.24–74.33 | 0.01–1407.10 | 0.03* |
IL-23 | 1.41 | DOWN | 0.14–3.39 | 0.01–7.63 | 0.42 |
IFN-γ | 1.03 | UP | 0.15–12.15 | 0.04–80.73 | 0.97 |
Eotaxin-2 | 3.49 | UP | 0.31–35.10 | 0.12–2104.64 | 0.053 |
TNF-α | 1.01 | DOWN | 0.32–3.41 | 0.07–8.24 | 0.99 |