The immune response of young turkeys to haemorrhagic enteritis virus infection at different levels and sources of methionine in the diet

Table 7 Experiment layout

Dietary treatment¹ (n = 60)	Group (n = 30)	Days of age
Dietary treatment¹ (n = 60)	Group (n = 30)	10	28	42 (experimental infection)	47	49	56
DLM_L	0-DLM_L	Vaccination against ND	Vaccination against ORT	0²	Collection of samples of blood and organs from 7 turkeys per group for immunological (CD4⁺, CD8⁺, CD4⁺CD8⁺, CD4⁺CD25⁺, CD4⁺IL-6⁺,IgM⁺) and biochemical analyses (IgA, IL-6)	Vaccination against ORT	Collection of blood samples from 23 turkeys per group for serological analyses (antibody against ORT and NDV)
DLM_L	HE-DLM_L			+ ³
MHA_L	0-MHA_L			0
MHA_L	HE-MHA_L			+
DLM_H	0-DLM_H			0
DLM_H	HE-DLM_H			+
MHA_H	0-MHA_H			0
MHA_H	HE-MHA_H			+

¹ Feeds administered to turkeys in weeks 1–4 and 5–8, contained DLM and the equivalent amount of MHA at two levels: low (_L) - 0.55, 0.45, and high (_H) - 0.78, 0.65, respectively [% as fed
² 0 - uninfected turkeys (received 1 ml of sterile PBS into the crop)
³+ − turkeys inoculated with 1 ml of a suspension containing HEV at a dose of 10^4,3 EID_50, administered into the crop

ISSN: 1746-6148