Table 5 Technical settings of the CASA system AndroVision® version 1.0.0.5; 2012 for motility analysis of buck spermatozoa
From: Effect of temperature and time after collection on buck sperm quality
Variables | Settings |
|---|---|
Depth of sample chamber | 20 μm |
Temperature during analysis | 38 °C |
Total number of cells evaluated | 2000 spermatozoa |
Sperm recognition area | 10–100 μm2 |
Frame rate | 60 frames/s |
Pixel/μm | 1/0.54 |
Progressive motility | Every cell that is not “immotile” or “local motile” |
Immotile | VSL < 12.0 and ALH < 1.50 |
Local motility | VCL < 60.0 and VSL < 48.0 |
Circle motility | Radius > 9.0 and radius < 90.0 and rotation > 0.70 |
Slow motility | VCL < 90.0 |
Fast motility | others |