Fig. 1

Cytological features of Mycoleptodiscus indicus subcutaneous infection in an 8-year-old cat, modified Wright-Giemsa stain. a Pyogranulomatous inflammation with both intralesional and extracellular fungal elements (asterisk). There are septated hyphae with irregularly sized segments, thin non-staining uneven walls, and some rounded yeast-like forms. Note the central yeast with radiating spokes of hyphae at the arrow (hub and spoke pattern). b Segments vary markedly in size, including very long hyphae (asterisk) and individual rounded yeast-like structures (arrow). c Fungal shapes are markedly pleomorphic. Intracellular organisms within macrophages appear ghost-like with pallor and minimal internal structure (asterisk). There are many neutrophils and rare plasma cells. d Numerous intracellular non-staining ghost-like organism outlines are noted in a macrophage (asterisk), while irregular segment shape and size appear to be a hallmark of this organism. Scale bar = 10 μm