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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Functional characterization of canine wild type glucocorticoid receptor and an insertional mutation in a dog

Fig. 5

Expression and sensitivity of cGRΔLBD to prednisolone. a COS-7 cells were transiently transfected with pCR3-cGR#3 or a cGRΔLBD-expressing vector. Forty-eight hours after transfection, whole cell lysates were prepared and analyzed by western blotting using an anti-FLAG antibody (M2). The results of 1 of 2 comparable experiments are shown. b, c Lower responsiveness of cGRΔLBD to prednisolone. COS-7 cells were transiently transfected with pGL4.36, pRL-SV40, and either a cGR- or cGRΔLBD-expressing vector and then treated with different doses of prednisolone (10− 4 to 1 μM) for 24 h. Transactivation of cGR (b) or cGRΔLBD (c) was evaluated using a luciferase assay. Values were calculated as % of maximal effect (b) or fold-increase in reactivity to basal treatment (c) and represent the means ±SE of more than three independent experiments

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