Development and application of an antibody detection ELISA for Haemophilus parasuis based on a monomeric autotransporter passenger domain

Table 1 Serum samples from vaccinated and experimentally infected pigs

	Vaccination or infection (No.)	Pigs with signs and lesions following challenge (No.)	Pigs without signs or lesions following challenge (No.)
Pig test 1	PBS control (15)	12 ^a	8
	Commercial killed vaccine (15)	3	12 ^b
	H. parasuis 011D (4)	0	4 ^c
Pig test 2	PBS control (4)	3	1
	Commercial killed vaccine (4)	1	3^d
	H. parasuis 016B (4)	0	4 ^e
	H. parasuis 014H (4)	0	4 ^f

^a The 12 serum samples were used as negative sera of H. parasuis for screening of antigens
^b The 12 serum samples were used as positive sera of H. parasuis for screening of antigens
^{b, d} The 15 serum samples from two groups were used as positive sera of H. parasuis for evaluation of positive percent agreement of the rApd ELISA
^{c, e, f} These 12 serum samples from three groups were used as positive sera of H. parasuis for evaluation of positive percent agreement of the rApd ELISA

ISSN: 1746-6148