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Table 4 Effects of RFI line and hygiene of housing conditions on antioxidant enzymes and glutathione content in tissues of growing pigs

From: Tissue-specific responses of antioxidant pathways to poor hygiene conditions in growing pigs divergently selected for feed efficiency

Line

Low RFI

High RFI

P values

Hygiene

Good

Poor

Good

Poor

MSE

Line

Hygiene

LxH

Week 6 (n = 36)

 SOD

  PRAT

81.4

109.4

99.5

104.8

26.1

0.44

0.06

0.17

  Liver

2727

2289

2929

2520

551

0.25

0.03

0.94

  LL

203

201

224

218

29.8

0.07

0.68

0.82

 CAT

  PRAT

1002

1299

1169

1500

258

0.04

0.001

0.84

  Liver (× 103)

72.3

74.3

88.0

71.6

25.1

0.44

0.40

0.28

  LL

1060

1116

1048

1020

193

0.41

0.83

0.52

 GSH-Rx

  PRAT

77.9

107.1

110.5

133.4

20

< 0.001

< 0.001

0.64

  Liver

5518

5214

5007

5098

631

0.16

0.63

0.37

  LL

121

102

116

117

23.6

0.53

0.29

0.23

GSH - Liver

5389

6202

5318

5122

1433

0.68

0.91

0.34

GSSG - Liver

275

286

283

257

162

0.83

0.88

0.77

Ratio - Liver

23.7

29.2

26.4

21.9

14.8

0.66

0.93

0.35

Week 13–14 (n = 35)

 SOD

  PRAT

42.4

53.9

53.4

82.5

18.9

0.004

0.004

0.18

  Liver

2594b

2392a

2507b

3257c

661

0.09

0.24

0.05

  LL

221

218

241

240

30

0.05

0.85

0.89

 CAT

  PRAT

781

810

812

1107

293

0.11

0.11

0.19

  Liver (× 103)

834

961

1004

923

180

0.29

0.72

0.10

  LL

1006

960

852

945

200

0.23

0.73

0.31

 GSH-Rx

  PRAT

52.2

67.9

59.8

89.3

23

0.07

0.007

0.38

  Liver

4334

4783

5066

5391

672

0.007

0.11

0.78

  LL

98

100

103

105

18

0.42

0.83

0.92

  1. Pigs from two genetic lines divergently selected for low (LRFI) or high (HRFI) residual feed intake (RFI) were housed either in good or poor hygiene conditions during the first 6 weeks after their transfer in growing-finishing pens (period 1, challenge). Half of these pigs were killed at week 6, whereas another half were placed in good hygiene conditions until slaughter at weeks 13 or 14 (period 2, recovery). Activities of antioxidant enzymes such as glutathione reductase (GSH-Rx), superoxide dismutase (SOD) and catalase (CAT) were assessed (nM/min/g tissue) in perirenal adipose tissue (PRAT), liver and longissimus lumborum (LL) muscle. Reduced (GSH) and oxidized (GSSG) forms of glutathione (expressed as pmol per well) were also assessed in the liver. The ratio between reduced and oxidized forms (GSH:GSSG) was calculated. Letters (a,b,c) were added in case of interaction (LxH; P < 0.10) between hygiene (H) and RFI line (L), and means sharing a common letter did not differ. MSE: root mean standard error of the statistical model. Bold face highlights significant differences (P ≤ 0.05) between treatments, and when italicized, this denotes a trend (0.05 < P ≤ 0.10)