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Table 3 IHC antibody specifications

From: Paving the way for more precise diagnosis of EcPV2-associated equine penile lesions

Primary antibody

Use

Retrieval

2nd antibody

Chromogen

Control

Monoclonal mouse anti-Ki67 (clone MIB-1; Dako)

1:50

1 h, RT

98 °C; pH 9

REAL kit

(Dako, K5007)

AEC

Equine skin, rectum

Monoclonal mouse anti-p53 (clone DO1; Santa Cruz)

1:100

1 h, RT

98 °C; pH 9

Envision mouse (Dako, K4001)

DAB

Equine SCC

Monoclonal mouse anti-MCM7 (clone DCS-141.2; Santa Cruz)

1:400

1 h, RT

98 °C; pH 9

Envision mouse (Dako, K4001)

DAB

Equine lymph node

Polyclonal rabbit anti-MMP1 (ThermoFisher)

1:50

O/N, 4 °C

98 °C; pH 7

Envision rabbit (Dako, K4003)

DAB

Equine SCC

  1. RT Room temperature, O/N overnight, AEC Aminoethylcarbazol, DAB Diaminobenzidine