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Table 3 IHC antibody specifications

From: Paving the way for more precise diagnosis of EcPV2-associated equine penile lesions

Primary antibody Use Retrieval 2nd antibody Chromogen Control
Monoclonal mouse anti-Ki67 (clone MIB-1; Dako) 1:50
1 h, RT
98 °C; pH 9 REAL kit
(Dako, K5007)
AEC Equine skin, rectum
Monoclonal mouse anti-p53 (clone DO1; Santa Cruz) 1:100
1 h, RT
98 °C; pH 9 Envision mouse (Dako, K4001) DAB Equine SCC
Monoclonal mouse anti-MCM7 (clone DCS-141.2; Santa Cruz) 1:400
1 h, RT
98 °C; pH 9 Envision mouse (Dako, K4001) DAB Equine lymph node
Polyclonal rabbit anti-MMP1 (ThermoFisher) 1:50
O/N, 4 °C
98 °C; pH 7 Envision rabbit (Dako, K4003) DAB Equine SCC
  1. RT Room temperature, O/N overnight, AEC Aminoethylcarbazol, DAB Diaminobenzidine