Fig. 3

p53, MCM7 and Ki67 immunostaining of penile lesions. One representative example per type of lesion (perilesional normal epithelium #14a, hyperplasia #14, papilloma #12, CIS #6 and SCC #3) is represented. p53, MCM7 and Ki67 immunostaining was photographed using the 10x objective. p53 immunostaining in perilesional epithelium and hyperplasia is restricted mainly to the basal layer with nuclear labelling of few/single cells. In papilloma, CIS and SCC almost all basal cells express p53. However, in CIS and SCC p53 expression extended to the upper suprabasal layers and was enhanced. MCM7 is expressed in almost all basal cells within all lesional and perilesional tissue samples. In CIS and SCC, MCM7 is also expressed in upper suprabasal layers of the epithelium. Ki67 was restricted mainly to the basal layer with nuclear labelling of few/single cells of perilesional epithelium, hyperplasia and papilloma. In malignant lesions Ki67 expression was increased and extended to upper suprabasal layers