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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017: recurrence of the CPV-2b variant

Fig. 2

Phylogenetic trees constructed on the VP2 nucleotide and amino acid sequence types obtained in this study. A coalescent constant population tree was estimated for both the ntSTs and aaSTs obtained in this study using BEAST 1.10.4 and BEAGLE 3.1.0 software. The Tamura 93 model with gamma distribution and the Jones-Taylor-Thornton (JTT) model with gamma distribution were used to build the ntST and the aaST phylogenetic trees, respectively. Phylogenetic analysis was carried out using the “strict clock” as a clock model for both nucleotide and amino acid sequence types. The Bayesian Markov chain Monte Carlo (MCMC) chain lengths were 40,000,000 generations, with sampling every 2000 generations. The tree iteration was discharged with 10% of the chains as a burn-in pattern by using a tree annotator, and the resulting MCMC tree was drawn using FigTree software. 1.4.2. Nucleotide STs and aaSTs were represented by points. In the ntST tree, the taxons were represented by points. In the aaST tree, the most important sequence types were in bold

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