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Table 1 Effects of three different Senna plants leaf extracts on Paramphistomum gracile in the unit and specific activity of tegumental enzymes (AcPase, AlkPase, ATPase, 5′-Nu, and AChE)

From: An in vitro confirmation of the ethonopharmacological use of Senna plants as anthelmintic against rumen fluke Paramphistomum gracile

  Control Senna alata Senna occidentalis Senna alexandrina
AcPase
 Unit activity 20.28 ± 2.68a 10.35 ± 0.69b 12.51 ± 2.94b 14.23 ± 3.35b
 Specific activity 0.65 ± 0.08a 0.30 ± 0.01d 0.36 ± 0.08c 0.43 ± 0.09b
 Inhibition in % 0 53.9 44.62 33.85
AlkPase
 Unit activity 343.97 ± 5.67a 175.55 ± 2.12d 187.78 ± 2.98c 203.63 ± 6.9b
 Specific activity 1.09 ± 0.02a 0.54 ± 0.01c 0.55 ± 0.02c 0.62 ± 0.01b
 Inhibition in % 0 50.5 49.54 43.12
ATPase
 Unit activity 936.22 ± 20.15a 627.82 ± 27.52d 782.24 ± 23.97c 869.76 ± 31.52b
 Specific activity 2.98 ± 0.06a 1.94 ± 0.05d 2.23 ± 0.06c 2.55 ± 0.18b
 Iniabition in % 0 34.9 25.17 14.43
5′- Nu
 Unit activity 1581 ± 25.66a 1159.5 ± 101.79a 1525.05 ± 33.49b 1573.95 ± 4.5a
 Specific activity 5.03 ± 0.08a 3.54 ± 0.31c 4.17 ± 0.01b 4.76 ± 0.01a
 Inhibition in % 0 29.6 17.10 5.37
AChE
 Unit activity 473.82 ± 21.55a 397.06 ± 51.45a 412.06 ± 55.33a 433.23 ± 54.69a
 Specific activity 11.66 ± 1.11a 9.41 ± 1.22b 9.57 ± 1.3b 11.39 ± 1.42a
 Inhibition in % 0 19.3 18.02 2.32
  1. Values are given as mean ± SE from five replicates assays (n = 5). Unit enzyme activity expressed as a specific unit which consumes 1.0 M substrate/g wet weight tissue/h. Specific activity = unit activity/mg tissue protein /h. Different small letters (a, b, c, d) on the error value indicate significant at (P < 0.001) among the treated worm as well as with the control. Same letters indicate no significant differences between the same variable between different plant extracts treatment
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