Skip to main content

Table 1 Effects of three different Senna plants leaf extracts on Paramphistomum gracile in the unit and specific activity of tegumental enzymes (AcPase, AlkPase, ATPase, 5′-Nu, and AChE)

From: An in vitro confirmation of the ethonopharmacological use of Senna plants as anthelmintic against rumen fluke Paramphistomum gracile

 

Control

Senna alata

Senna occidentalis

Senna alexandrina

AcPase

 Unit activity

20.28 ± 2.68a

10.35 ± 0.69b

12.51 ± 2.94b

14.23 ± 3.35b

 Specific activity

0.65 ± 0.08a

0.30 ± 0.01d

0.36 ± 0.08c

0.43 ± 0.09b

 Inhibition in %

0

53.9

44.62

33.85

AlkPase

 Unit activity

343.97 ± 5.67a

175.55 ± 2.12d

187.78 ± 2.98c

203.63 ± 6.9b

 Specific activity

1.09 ± 0.02a

0.54 ± 0.01c

0.55 ± 0.02c

0.62 ± 0.01b

 Inhibition in %

0

50.5

49.54

43.12

ATPase

 Unit activity

936.22 ± 20.15a

627.82 ± 27.52d

782.24 ± 23.97c

869.76 ± 31.52b

 Specific activity

2.98 ± 0.06a

1.94 ± 0.05d

2.23 ± 0.06c

2.55 ± 0.18b

 Iniabition in %

0

34.9

25.17

14.43

5′- Nu

 Unit activity

1581 ± 25.66a

1159.5 ± 101.79a

1525.05 ± 33.49b

1573.95 ± 4.5a

 Specific activity

5.03 ± 0.08a

3.54 ± 0.31c

4.17 ± 0.01b

4.76 ± 0.01a

 Inhibition in %

0

29.6

17.10

5.37

AChE

 Unit activity

473.82 ± 21.55a

397.06 ± 51.45a

412.06 ± 55.33a

433.23 ± 54.69a

 Specific activity

11.66 ± 1.11a

9.41 ± 1.22b

9.57 ± 1.3b

11.39 ± 1.42a

 Inhibition in %

0

19.3

18.02

2.32

  1. Values are given as mean ± SE from five replicates assays (n = 5). Unit enzyme activity expressed as a specific unit which consumes 1.0 M substrate/g wet weight tissue/h. Specific activity = unit activity/mg tissue protein /h. Different small letters (a, b, c, d) on the error value indicate significant at (P < 0.001) among the treated worm as well as with the control. Same letters indicate no significant differences between the same variable between different plant extracts treatment