Table 5 Primer sets for Bruce ladder multiplex PCR
Primer | Sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|
BMEI0998f BMEI0997r | ATC-CTA-TTG-CCC-CGA-TAA-GG GCT-TCG-CAT-TTT-CAC-TGT-AGC | 1682 |
BMEII0843f BMEII0844r | TTT-ACA-CAG-GCA-ATC-CAG-CA GCG-TCC-AGT-TGT-TGT-TGA-TG | 1071 |
BMEII0428f BMEII0428r | GCC-GCT-ATT-ATG-TGG-ACT-GG AAT-GAC-TTC-ACG-GTC-GTT-CG | 587 |
BR0953f BR0953r | GGA-ACA-CTA-CGC-CAC-CTT-GT GAT-GGA-GCA-AAC-GCT-GAA-G | 272 |
BMEI0752f BMEI0752r | CAG-GCA-AAC-CCT-CAG-AAG-C GAT-GTG-GTA-ACG-CAC-ACC-AA | 218 |