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Table 2 Intra- and inter-assay reproducibility for TaqMan qPCR

From: Specific detection and differentiation of classic goose parvovirus and novel goose parvovirus by TaqMan real-time PCR assay, coupled with host specificity

Concentration of standard plasmid (copies/μl)

Intra-assay variability

Inter-assay variability

\( \overline{X}\pm SD \)

CV (%)

\( \overline{X}\pm SD \)

CV (%)

5.02 × 105

18.17 ± 0.11

0.58

18.19 ± 0.12

0.66

5.02 × 103

24.56 ± 0.19

0.78

24.69 ± 0.22

0.90

5.02 × 101

31.55 ± 0.55

1.74

31.71 ± 0.75

2.37