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Table 1 The list of primer used in the experiment

From: Immune inhibitory function of bovine CTLA-4 and the effects of its blockade in IFN-γ production

Primer

Primer sequences (5'-3')

Restriction enzyme

BLV diagnosis

 BLV-LTR-1

TGTATGAAAGATCATGCCGAC

 

 BLV-LTR-533

AATTGTTTGCCGGTCTCT

 

 BLV-LTR-256

GAGCTCTCTTGCTCCCGAGAC

 

 BLV-LTR-453

GAAACAAACGCGGGTGCAAGCCAG

 

 β-globin Forward (F)

ACACAACTGTGTTCACTAGC

 

 β-globin Reverse (R )

CAACTTCATCCACGTTCACC

 

Establishment of expression vector

 pCXN2.1-CTLA-4-Ig F

CGCGGATATCATGGCTTGCTCTGGATTCCA

EcoRV

 pCXN2.1-CTLA-4-Ig R

CGGGGTACCATCAGAATCCGGGCATGGTT

KpnI-HF

 pCXN2.1-CD80-Ig F

CGCGGATATCATGGGTCACACAATGAAGTG

EcoRV

 pCXN2.1-CD80-Ig R

CGGGGTACCGGTCCAGGTCAGGTGCTGAT

KpnI-HF

 pCXN2.1-CD86-Ig F

CGCGGATATCATGCGTTTCAAATGCACCAT

EcoRV

 pCXN2.1-CD86-Ig R

CGGGGTACCTGGGACAGGGGGGCTTGGCA

KpnI-HF

 pEGFP-N2-CTLA-4 F

GGAAGATCTATGGCTTGCTCTGGATTCCA

BglII

 pEGFP-N2-CTLA-4 R

CCGGAATTCATTGATGGGAATAAAATAAG

EcoRI

 pEGFP-N2-CD28 F

GGAAGATCTATGCTCAGGCTGCTCCTGGC

BglII

 pEGFP-N2-CD28 R

TCCCCCGGGGGAGCGGTAGGCCGCAAAGT

SmaI

  1. *Recognition sites of restriction enzymes were underlined