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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of an indirect ELISA for detecting humoral immunodominant proteins of Mycoplasma hyopneumoniae which can discriminate between inactivated bacterin-induced hyperimmune sera and convalescent sera

Fig. 2

Optimization of ELISA working condition. a The optimal concentration of antigen was the bacterial lysates of fusion protein and free GST protein without dilution. b The optimal blocking buffer was PBS comprising 10% FBS and 2.5% skimmed milk. c Both hyperimmune serum and convalescent serum with dilution of 1:500 and HRP-labeled rabbit anti-pig IgG diluted at 1:20000 were the working concentration of primary antibodies and secondary antibody

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BMC Veterinary Research

ISSN: 1746-6148

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